Human Transcriptional repressor CTCFL ELISA Kit | CTCFL elisa kit

Human Transcriptional repressor CTCFL ELISA Kit

Cat. Num. AAA763991

• Gene Names: Ctcfl; Boris; OTTMUSG00000016680
• Reactivity: Human
• Synonyms: Transcriptional repressor CTCFL; Human Transcriptional repressor CTCFL ELISA Kit; CTCFL/Zinc finger protein CTCF-T/Cancer/testis antigen 27 (CT27)/CTCF-like protein/CTCF paralog/Brother of the regulator of imprinted sites/CCCTC-binding factor; CTCFL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CTCFL . No significant cross-reactivity or interference between CTCFL and analogues was observed.
• Sequence Length: 636

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CTCFL were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CTCFL were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTCFL elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CTCFL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CTCFL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CTCFL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CTCFL can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 124487069
• NCBI GeneID: 664799
• NCBI Accession #: NP_001074856.1
• NCBI GenBank Nucleotide #: NM_001081387.2
• Molecular Weight: 74,098 Da
• NCBI Official Full Name: transcriptional repressor CTCFL
• NCBI Official Synonym Full Names: CCCTC-binding factor (zinc finger protein)-like
• NCBI Official Symbol: Ctcfl
• NCBI Official Synonym Symbols: Boris; OTTMUSG00000016680
• NCBI Protein Information: transcriptional repressor CTCFL
• UniProt Protein Name: Transcriptional repressor CTCFL
• Protein Family: Transcriptional repressor
• UniProt Gene Name: Ctcfl
• UniProt Synonym Gene Names: Boris
• UniProt Entry Name: CTCFL_MOUSE

Uniprot Description

BORIS: Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites. Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters. Interacts with histones, PRMT7 and SETD1A. Interacts (via N-terminus) with BAG6/BAT3. Testis specific. Specifically expressed in primary spermatocytes. Belongs to the CTCF zinc-finger protein family.

Protein type: DNA-binding; Cancer Testis Antigen (CTA); C2H2-type zinc finger protein

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA binding; histone binding; metal ion binding; nucleic acid binding; protein binding; sequence-specific DNA binding

Biological Process: chromatin modification; DNA methylation during gametogenesis; genetic imprinting; histone methylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of histone H3-K4 methylation; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter; transcription, DNA-dependent