Human Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4) ELISA Kit | HCN4 elisa kit

Human Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4) ELISA Kit

Cat. Num. AAA2706549

• Gene Names: HCN4; SSS2
• Reactivity: Human
• Synonyms: Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4); Human Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4) ELISA Kit; HCN4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4).; No significant cross-reactivity or interference between Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4) and analogues was observed.
• Sequence Length: 1203

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.122ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HCN4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4 (HCN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119598331
• NCBI GeneID: 10021
• Molecular Weight: 129,042 Da
• NCBI Official Full Name: hyperpolarization activated cyclic nucleotide-gated potassium channel 4
• NCBI Official Synonym Full Names: hyperpolarization activated cyclic nucleotide gated potassium channel 4
• NCBI Official Symbol: HCN4
• NCBI Official Synonym Symbols: SSS2
• NCBI Protein Information: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
• UniProt Protein Name: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
• Protein Family: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel
• UniProt Gene Name: HCN4

NCBI Description

This gene encodes a member of the hyperpolarization-activated cyclic nucleotide-gated potassium channels. The encoded protein shows slow kinetics of activation and inactivation, and is necessary for the cardiac pacemaking process. This channel may also mediate responses to sour stimuli. Mutations in this gene have been linked to sick sinus syndrome 2, also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. Two pseudogenes have been identified on chromosome 15. [provided by RefSeq, Oct 2008]

Uniprot Description

HCN4: Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli. Defects in HCN4 are a cause of sick sinus syndrome type 2 (SSS2); also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors, in which case it is considered to be a congenital disorder. Defects in HCN4 are the cause of Brugada syndrome type 8 (BRGDA8). A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the potassium channel HCN family.

Protein type: Channel, cation; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: intrinsic component of plasma membrane; perinuclear region of cytoplasm; plasma membrane

Molecular Function: cAMP binding; identical protein binding; intracellular cAMP activated cation channel activity; voltage-gated potassium channel activity; voltage-gated sodium channel activity

Biological Process: blood circulation; cation transport; muscle contraction; regulation of heart rate; regulation of membrane potential; regulation of postsynaptic membrane potential

Disease: Brugada Syndrome 8; Sick Sinus Syndrome 2, Autosomal Dominant