Sequence Information
Granzyme M (GZMM) Recombinant Protein | GZMM recombinant protein
Recombinant Granzyme M (GZMM)
Gene Names
Gzmm; Lmet1; MMET-1
Reactivity
Mus musculus (Mouse)
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Granzyme M (GZMM); Recombinant Granzyme M (GZMM); GZMM recombinant protein
Host
E.coli
Reactivity
Mus musculus (Mouse)
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 100ug/mL (varies by lot)
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- REAVPHSRPY MASLQKAKSH VCGGVLVHRK WVLTAAHCLS EPLQNLKLVL GLHNLHDLQD PGLTFYIREA IKHPGYNHKY ENDLALLKLD RRVQPSKNVK PLALPRKPRS KPAEGTWCST AGWGMTHQGG PRARALQELD LRVLDTQMCN NSRFWNGVLI DSMLCLKAGS KSQAPCKGDS GGPLVCGKGQ VDGILSFSSK TCTDIFKPPV ATAVAPYSSW IRKVIGR
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- REAVPHSRPY MASLQKAKSH VCGGVLVHRK WVLTAAHCLS EPLQNLKLVL GLHNLHDLQD PGLTFYIREA IKHPGYNHKY ENDLALLKLD RRVQPSKNVK PLALPRKPRS KPAEGTWCST AGWGMTHQGG PRARALQELD LRVLDTQMCN NSRFWNGVLI DSMLCLKAGS KSQAPCKGDS GGPLVCGKGQ VDGILSFSSK TCTDIFKPPV ATAVAPYSSW IRKVIGR
Sequence Length
264
Applicable Applications for GZMM recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Arg31~Arg257
Tags
N-terminal His and GST Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
8.9
Usage
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
Sequence Information
Sequence Information
SDS-Page
SDS-Page
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 55.0kDa
Accurate Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
granzyme M
NCBI Official Synonym Full Names
granzyme M (lymphocyte met-ase 1)
NCBI Official Symbol
Gzmm
NCBI Official Synonym Symbols
Lmet1; MMET-1
NCBI Protein Information
granzyme M; lymphoctye Met-ase 1
UniProt Protein Name
Granzyme M
Protein Family
UniProt Gene Name
Gzmm
UniProt Synonym Gene Names
LMet-1
UniProt Entry Name
O08643_MOUSE
NCBI Description
The protein encoded by this gene is a member of a family of cytotoxic lymphocyte serine proteases called granzymes, which are expressed by cytotoxic T lymphocytes and natural killer cells. This protein belongs to a subfamily of granzymes that cleave after methionine residues. Natural killer cell development, homeostasis and cytotoxicity are normal in mice deficient for this gene, but they demonstrate increased susceptibility to murine cytomegalovirus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Uniprot Description
GZMM: Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha- tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells. Belongs to the peptidase S1 family. Granzyme subfamily.
Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.21.-
Cellular Component: membrane
Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity; endopeptidase activity
Biological Process: cell death; cytolysis; proteolysis
Research Articles on GZMM
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Product Notes
The GZMM gzmm (Catalog #AAA2009188) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Granzyme M (GZMM) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Granzyme M (GZMM) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the GZMM gzmm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF - REAVPHSRPY MASLQKAKSH VCGGVLVHRK WVLTAAHCLS EPLQNLKLVL GLHNLHDLQD PGLTFYIREA IKHPGYNHKY ENDLALLKLD RRVQPSKNVK PLALPRKPRS KPAEGTWCST AGWGMTHQGG PRARALQELD LRVLDTQMCN NSRFWNGVLI DSMLCLKAGS KSQAPCKGDS GGPLVCGKGQ VDGILSFSSK TCTDIFKPPV ATAVAPYSSW IRKVIGR. It is sometimes possible for the material contained within the vial of "Granzyme M (GZMM), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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