Granzyme M (GZMM) Recombinant Protein | GZMM recombinant protein

Recombinant Granzyme M (GZMM)

Cat. Num. AAA2011633

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Granzyme M (GZMM); Recombinant Granzyme M (GZMM); GZMM recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-AQ IIGGREAVPH SRPYMVSLQN TKSHMCGGVL VHQKWVLTAA HCLSEPLQQL KLVFGLHSLH DPQDPGLTFY IKQAIKHPGY NLKYENDLAL LKLDGRVKPS KNVKPLALPR KPRDKPAEGS RCSTAGWGIT HQRGQLAKSL QELDLRLLDT RMCNNSRFWN GVLTDSMLCL KAGAKGQAPC KGDSGGPLVC GKGKVDGILS FSSKNCTDIF KPTVATAVAP YSSWIRKVIG R
• Sequence Length: 258

• Applicable Applications for GZMM recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ala19~Arg251 (Accession # Q03238) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Secreted. Cytoplasmic granule
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.9
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 548367
• UniProt Accession #: Q03238
• Molecular Weight: 57.4kDa
• NCBI Official Full Name: Granzyme M
• UniProt Protein Name: Granzyme M
• Protein Family: Granzyme
• UniProt Gene Name: Gzmm
• UniProt Entry Name: GRAM_RAT

Uniprot Description

Function: Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha-tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells

By similarity.

Subcellular location: Secreted. Cytoplasmic granule. Note: Granules of large granular lymphocytes.

Sequence similarities: Belongs to the peptidase S1 family. Granzyme subfamily.Contains 1 peptidase S1 domain.