Guinea Pig PRL ELISA Kit | PRL elisa kit

Guinea pig PRL (Prolactin) ELISA Kit

Cat. Num. AAA2510019

• Reactivity: Guinea Pig
• Synonyms: PRL; Guinea pig PRL (Prolactin) ELISA Kit; PRL elisa kit

• Reactivity: Guinea Pig
• Specificity: This kit recognizes natural and recombinantGuinea pigPRL. No significant cross-reactivity or interference between Guinea pigPRL and analogues was observed.
• Sequence Length: 227

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL ; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRL elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of Guinea pigPRLconcentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PRL. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PRLand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PRL.You can calculate the concentration of PRLin the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 130930
• NCBI GeneID: 5617
• UniProt Accession #: P01236; Q15199; Q92996
• Molecular Weight: 25,876 Da
• NCBI Official Full Name: Prolactin
• NCBI Official Synonym Full Names: prolactin
• NCBI Official Symbol: PRL
• NCBI Protein Information: prolactin; decidual prolactin
• UniProt Protein Name: Prolactin
• UniProt Gene Name: PRL
• UniProt Synonym Gene Names: PRL
• UniProt Entry Name: PRL_HUMAN

NCBI Description

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Uniprot Description

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: extracellular region

Molecular Function: protein binding; prolactin receptor binding; hormone activity

Biological Process: lactation; cell proliferation; cell surface receptor linked signal transduction; positive regulation of JAK-STAT cascade; regulation of multicellular organism growth; female pregnancy