Canine Prolactin ELISA Kit | PRL elisa kit

Canine Prolactin ELISA Kit

Cat. Num. AAA734567

• Reactivity: Canine
• Synonyms: Prolactin; Canine Prolactin ELISA Kit; PRL elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRL elisa kit

Intended Uses: This PRL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PRL. This ELISA kit for research use only!

Principle of the Assay: PRL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PRL antibody and an PRL-HRP conjugate. The assay sample and buffer are incubated together with PRL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRL concentration since PRL from samples and PRL-HRP conjugate compete for the anti-PRL antibody binding site. Since the number of sites is limited, as more sites are occupied by PRL from the sample, fewer sites are left to bind PRL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRL concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 130930
• NCBI GeneID: 5617
• Molecular Weight: 25,876 Da
• NCBI Official Full Name: Prolactin
• NCBI Official Synonym Full Names: prolactin
• NCBI Official Symbol: PRL
• NCBI Protein Information: prolactin; decidual prolactin
• UniProt Protein Name: Prolactin
• Protein Family: Prolactin
• UniProt Gene Name: PRL
• UniProt Synonym Gene Names: PRL
• UniProt Entry Name: PRL_HUMAN

NCBI Description

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Uniprot Description

prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.

Protein type: Motility/polarity/chemotaxis; Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: extracellular region

Molecular Function: protein binding; prolactin receptor binding; hormone activity

Biological Process: lactation; cell proliferation; cell surface receptor linked signal transduction; regulation of multicellular organism growth; positive regulation of JAK-STAT cascade; female pregnancy