Guinea Pig DPD ELISA Kit | DPD elisa kit
Guinea pig DPD (Deoxypyridinoline) ELISA Kit
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with DPD. During the reaction, DPD in the sample or standard competes with a fixed amount of DPD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DPD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DPD in the samples is then determined by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
catalyzes the conversion of 5,6-dihydrouracil and NADP+ to uracil, NADPH, and H+ in pyrimidine degradation [RGD, Feb 2006]
Uniprot Description
DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family.
Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Other Amino Acids Metabolism - beta-alanine; EC 1.3.1.2; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Nucleotide Metabolism - pyrimidine; Oxidoreductase
Cellular Component: cytoplasm; cytosol
Molecular Function: protein homodimerization activity; uracil binding; dihydroorotate dehydrogenase activity; metal ion binding; 4 iron, 4 sulfur cluster binding; iron-sulfur cluster binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding
Biological Process: uracil metabolic process; circadian rhythm; response to drug; uracil catabolic process; UMP biosynthetic process; response to glucocorticoid stimulus; thymine catabolic process; beta-alanine biosynthetic process; purine base catabolic process; response to nutrient; thymidine catabolic process; pyrimidine base catabolic process
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Product Notes
The Guinea Pig DPD dpyd (Catalog #AAA2511347) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2511347 ELISA Kit recognizes Guinea Pig DPD. It is sometimes possible for the material contained within the vial of "DPD, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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