Rabbit deoxypyridinoline (DPD) ELISA Kit | DPD elisa kit

Rabbit deoxypyridinoline (DPD) ELISA Kit

Cat. Num. AAA2600776

• Gene Names: DPYD; DHP; DPD; DHPDHASE
• Reactivity: Rabbit
• Synonyms: deoxypyridinoline (DPD); Rabbit deoxypyridinoline (DPD) ELISA Kit; DPD elisa kit

• Reactivity: Rabbit
• Sequence Length: 1025

• Samples: Rabbit serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 200 ng/mL-3.12 ng/mL
• Sensitivity: Up to 0.6 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPD elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rabbit DPD monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 160332325
• NCBI GeneID: 1806
• Molecular Weight: 18,936 Da
• NCBI Official Full Name: Dihydropyrimidine dehydrogenase
• NCBI Official Synonym Full Names: dihydropyrimidine dehydrogenase
• NCBI Official Symbol: DPYD
• NCBI Official Synonym Symbols: DHP; DPD; DHPDHASE
• NCBI Protein Information: dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; dihydrouracil dehydrogenase; dihydrothymine dehydrogenase
• UniProt Protein Name: Dihydropyrimidine dehydrogenase [NADP(+)]
• UniProt Gene Name: DPYD
• UniProt Synonym Gene Names: DHPDHase; DPD
• UniProt Entry Name: DPYD_HUMAN

NCBI Description

The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family.

Protein type: Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Xenobiotic Metabolism - drug metabolism - other enzymes; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase; EC 1.3.1.2

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: cytoplasm; cytosol

Molecular Function: protein homodimerization activity; FAD binding; 4 iron, 4 sulfur cluster binding; dihydroorotate dehydrogenase activity; metal ion binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; uracil catabolic process; UMP biosynthetic process; beta-alanine biosynthetic process; thymine catabolic process; purine base catabolic process; thymidine catabolic process; pyrimidine base catabolic process

Disease: Dihydropyrimidine Dehydrogenase Deficiency