Mouse DPD ELISA Kit | DPD elisa kit

Mouse DPD (Deoxypyridinoline) ELISA Kit

Cat. Num. AAA2503693

• Reactivity: Mouse
• Synonyms: DPD; Mouse DPD (Deoxypyridinoline) ELISA Kit; DPD elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes Mouse DPD in samples. No significant cross-reactivity or interference between Mouse DPD and analogues was observed.
• Sequence Length: 1025

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 3.13-200ng/mL
• Sensitivity: 1.88ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse DPD were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse DPD were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPD elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse DPD concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse DPD. During the reaction, Mouse DPD in the sample or standard competes with a fixed amount of Mouse DPD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse DPD. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Mouse DPD in the samples is then determined by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13591940
• NCBI GeneID: 81656
• NCBI Accession #: NP_112289.1
• NCBI GenBank Nucleotide #: NM_031027.1
• UniProt Accession #: O89000
• Molecular Weight: 111,468 Da
• NCBI Official Full Name: dihydropyrimidine dehydrogenase
• NCBI Official Synonym Full Names: dihydropyrimidine dehydrogenase
• NCBI Official Symbol: Dpyd
• NCBI Protein Information: dihydropyrimidine dehydrogenase [NADP(+)]; dihydropyrimidine dehydrogenase [NADP(+)]; DHPDHase; DPD; dihydrothymine dehydrogenase; dihydrouracil dehydrogenase
• UniProt Protein Name: Dihydropyrimidine dehydrogenase [NADP(+)]
• UniProt Gene Name: Dpyd
• UniProt Synonym Gene Names: DPD; DHPDHase; DPD
• UniProt Entry Name: DPYD_RAT

NCBI Description

catalyzes the conversion of 5,6-dihydrouracil and NADP+ to uracil, NADPH, and H+ in pyrimidine degradation [RGD, Feb 2006]

Uniprot Description

DPYD: Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. Homodimer. Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. Belongs to the dihydropyrimidine dehydrogenase family.

Protein type: Other Amino Acids Metabolism - beta-alanine; Nucleotide Metabolism - pyrimidine; EC 1.3.1.2; Oxidoreductase; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Xenobiotic Metabolism - drug metabolism - other enzymes

Cellular Component: cytoplasm; cytosol

Molecular Function: protein homodimerization activity; uracil binding; dihydroorotate dehydrogenase activity; metal ion binding; 4 iron, 4 sulfur cluster binding; iron-sulfur cluster binding; dihydropyrimidine dehydrogenase (NADP+) activity; NADP binding

Biological Process: response to drug; uracil metabolic process; circadian rhythm; UMP biosynthetic process; uracil catabolic process; response to glucocorticoid stimulus; beta-alanine biosynthetic process; thymine catabolic process; purine base catabolic process; response to nutrient; thymidine catabolic process; pyrimidine base catabolic process