Human TRA2B (Transformer-2 Protein Homolog Beta) ELISA Kit | TRA2B elisa kit

Human TRA2B (Transformer-2 Protein Homolog Beta) ELISA Kit

Cat. Num. AAA7607000

• Gene Names: TRA2B; SFRS10; SRFS10; TRAN2B; PPP1R156; TRA2-BETA; Htra2-beta
• Reactivity: Human
• Synonyms: TRA2B (Transformer-2 Protein Homolog Beta); Human TRA2B (Transformer-2 Protein Homolog Beta) ELISA Kit; TRA2B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TRA2B. No significant cross-reactivity or interference between TRA2B and analogues was observed.
• Sequence Length: 188

• Samples: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: <0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TRA2B were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TRA2B were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TRA2B elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TRA2B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TRA2B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TRA2B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TRA2B can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 345197228
• NCBI GeneID: 6434
• NCBI Accession #: NP_001230808.1
• NCBI GenBank Nucleotide #: NM_001243879.1
• UniProt Accession #: P62995; O15449; Q15815; Q64283; B4DVK2; D3DNU3
• Molecular Weight: 21,935 Da
• NCBI Official Full Name: transformer-2 protein homolog beta isoform 2
• NCBI Official Synonym Full Names: transformer 2 beta homolog
• NCBI Official Symbol: TRA2B
• NCBI Official Synonym Symbols: SFRS10; SRFS10; TRAN2B; PPP1R156; TRA2-BETA; Htra2-beta
• NCBI Protein Information: transformer-2 protein homolog beta
• UniProt Protein Name: Transformer-2 protein homolog beta
• Protein Family: Transformer-2 protein
• UniProt Gene Name: TRA2B
• UniProt Synonym Gene Names: SFRS10; TRA-2 beta; TRA2-beta; hTRA2-beta

NCBI Description

This gene encodes a nuclear protein which functions as sequence-specific serine/arginine splicing factor which plays a role in mRNA processing, splicing patterns, and gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA.