Rabbit anti-Human, Mouse PEDF Polyclonal Antibody | anti-PEDF antibody

PEDF (Pigment Epithelial Derived Factor) (MaxLight 405)

Cat. Num. AAA6492617

• Gene Names: SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PEDF; Polyclonal Antibody; PEDF (Pigment Epithelial Derived Factor) (MaxLight 405); anti-PEDF antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PEDF at ~50kD. Additional bands were detected at 66 and 76kD, which were competed away by immunogen. These bands may be post-translationally modified forms. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-PEDF antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.; WB: 0.5-2ug/ml detects PEDF in 40ug of RIPA lysates from Y79 cells. 40ug of Y79 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with. Optimal dilutions to be determined by the researcher.
• Immunogen: His tagged fusion protein corresponding to full length human PEDF (Pigment Epithelial Derived Factor) expressed in baby hamster kidney cells.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of Y79 cell lysate probed with 1ug/ml of P3140.)

Related Product Information for anti-PEDF antibody

Pigment epithelium-derived factor (PEDF) was first identified as a 50kD secreted protein in conditioned medium from cultured fetal human retinal pigment epithelium (RPE) cells. More recently, PEDF was shown to have neurotrophic activity in the retina in vivo. PEDF is also a neurotrophic and neuroprotective factor in other systems, including cultured cerebellar granule cells, primary hippocampal neurons, spinal cord motor neurons and amphibian photoreceptors cultured in the absence of RPE cells. The binding of PEDF to retinoblastoma and cerebellar granule cells may be receptor-mediated. PEDF has been shown to be a potent inhibitor of angiogenesis in endothelial cell cultures and in rat cornea and retina in vivo.

Product Categories/Family for anti-PEDF antibody

Antibodies; Protease Inhibitors

References

1. Karakousis, P.C., et al., Molecular Vision 7: 154-163 (2001). 2. Tombran-Tink, J., et al., 1991, Exp. Eye Res., 53: 411-414. 3. Cayouette, M., et al., 1999, Neurobiol. Dis., 6: 523-532. 4. Karakousis, P.C., et al., 2001, Mol Vis., 7: 154-163. 5. Dawson, D.W., et al., 1999, Science, 285: 245-248. 6. Stellmach, V., et al., 2001, Proc. Natl. Acad. Sci. USA, 98: 2593-2597.

NCBI and Uniprot Product Information

• NCBI GI #: 39725934
• NCBI GeneID: 5176
• NCBI Accession #: NP_002606.3
• NCBI GenBank Nucleotide #: NM_002615
• UniProt Accession #: P36955; Q13236; Q2TU83; Q96CT1; Q96R01; Q9BWA4; F1T092
• Molecular Weight: 46,312 Da
• NCBI Official Full Name: pigment epithelium-derived factor
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• NCBI Official Symbol: SERPINF1
• NCBI Official Synonym Symbols: OI6; OI12; PEDF; EPC-1; PIG35
• NCBI Protein Information: pigment epithelium-derived factor; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• UniProt Protein Name: Pigment epithelium-derived factor
• UniProt Gene Name: SERPINF1
• UniProt Synonym Gene Names: PEDF; PEDF
• UniProt Entry Name: PEDF_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]

Uniprot Description

PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: axon hillock; extracellular matrix; extracellular space; perinuclear region of cytoplasm; extracellular region; melanosome; basement membrane

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; positive regulation of neurogenesis; negative regulation of angiogenesis; ovulation cycle; retina development in camera-type eye; short-term memory; response to arsenic; negative regulation of inflammatory response; multicellular organismal development; response to acidity; kidney development; aging

Disease: Osteogenesis Imperfecta, Type Vi