Western Blot (WB)
(The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEDF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: PEDF transfected 293T cell lysate)
Mouse anti-Human PEDF Monoclonal Antibody | anti-PEDF antibody
PEDF antibody
Gene Names
GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
By protein-A affinity chromatography
Synonyms
PEDF; Monoclonal Antibody; PEDF antibody; Pigment epithelium-derived factor; EPC-1; PIG35; SERPINF1; Pigment epithelium-derived factor EPC 1; EPC1; PIG 35; Pigment epithelium derived factor; Proliferation inducing protein 35; SERPIN F1; Serpin peptidase inhibitor clade F member 1; SERPINF 1; ; anti-PEDF antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT13D9
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
210
Applicable Applications for anti-PEDF antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Antigen Species
Human
Immunogen
Recombinant human PEDF (20-418aa) purified from E. coli.
Clone Note
Anti-human PEDF mAb, clone AT13D9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PEDF protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEDF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: PEDF transfected 293T cell lysate)
Western Blot (WB)
(The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEDF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: PEDF transfected 293T cell lysate)
Related Product Information for anti-PEDF antibody
Pigment epithelium-derived factor (PEDF) is a 50kDa glycoprotein and member of the serpin superfamily without any activity of serine protease. PEDF is an angiogenesis inhibitor with neurotrophic properties, balances angiogenesis in the eye and blocks tumor progression. That is, in neuronal cell, PEDF is promoting cell survival and differentiation, however, when endothelial cells are exposed to PEDF, their cell migration and proliferation are inhibited, and they undergo apoptosis.
Product Categories/Family for anti-PEDF antibody
References
Baba H. et al (2005) Arteriosler Thromb Vasc Biol. 25: 1938-1944.; Filleur S. et al (2005) Cancer Res. 65: 5144-5152.; Hattenbach LO. et al (2004) Ophthalmic Res. 37: 341-346.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,356 Da
NCBI Official Full Name
glutathione S-transferase P
NCBI Official Synonym Full Names
glutathione S-transferase pi 1
NCBI Official Symbol
GSTP1
NCBI Official Synonym Symbols
PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
NCBI Protein Information
glutathione S-transferase P
UniProt Protein Name
Glutathione S-transferase P
UniProt Gene Name
GSTP1
UniProt Synonym Gene Names
FAEES3; GST3
NCBI Description
Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]
Uniprot Description
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
Research Articles on PEDF
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Product Notes
The PEDF gstp1 (Catalog #AAA206600) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PEDF antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PEDF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the PEDF gstp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PEDF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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