GSTM2 / GST4 recombinant protein

Recombinant Human GSTM2 / GST4 Protein (His tag)

Cat. Num. AAA2545595

• Gene Names: GSTM2; GST4; GSTM; GTHMUS; GSTM2-2
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: GSTM2 / GST4; Recombinant Human GSTM2 / GST4 Protein (His tag); GST4; GSTM; GSTM2-2; GTHMUS; MGC117303; GSTM2 / GST4 recombinant protein

• Host: E Coli
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 150mM NaCl, 10mM CaCl2, 0.05% Brig-35, pH 7.5
• Sequence Length: 218

• Application Notes: The recombinant human GSTM2 consisting of 229 amino acids and has a calculated molecular mass of 27.2 kDa. It migrates as a 27 kDa band in SDS-PAGE under reducing conditions as predicted.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for GSTM2 / GST4 recombinant protein

Background: Glutathione S-transferase Mu 2, also known as GST class-mu 2, GSTM2-2 and GSTM2, is a cytoplasm protein which belongs to the GST superfamily and Mu family. GSTM2 / GST4 contains one GST C-terminal domain and one GST N-terminal domain. The glutathione S-transferases (GSTs) are a multigene family of enzymes largely involved in the detoxification of chemicals. Eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. Butyrate, an important luminal component produced from fermentation of dietary fibers, is an efficient inducer of GSTs and especially of GSTM2. Butyrate may act chemoprotectively by increasing detoxification capabilities in the colon mucosa.

Description: A DNA sequence encoding the native human GSTM2 (P28161) (Met 1-Lys 218) was expressed, with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4504175
• NCBI GeneID: 2946
• NCBI Accession #: NP_000839.1
• NCBI GenBank Nucleotide #: NM_000848.3
• UniProt Accession #: P28161; Q2M318; Q5TZY5; Q8WWE1; B4DRY4; E9PEM9
• Molecular Weight: 22,644 Da
• NCBI Official Full Name: glutathione S-transferase Mu 2 isoform 1
• NCBI Official Synonym Full Names: glutathione S-transferase mu 2
• NCBI Official Symbol: GSTM2
• NCBI Official Synonym Symbols: GST4; GSTM; GTHMUS; GSTM2-2
• NCBI Protein Information: glutathione S-transferase Mu 2
• UniProt Protein Name: Glutathione S-transferase Mu 2
• UniProt Gene Name: GSTM2
• UniProt Synonym Gene Names: GST4

NCBI Description

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. [provided by RefSeq, Jul 2008]

Uniprot Description

GSTM2: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family.

Protein type: EC 2.5.1.18; Motility/polarity/chemotaxis; Transferase

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytoplasm; cytosol; sarcoplasmic reticulum

Molecular Function: enzyme binding; glutathione binding; glutathione peroxidase activity; glutathione transferase activity; protein binding; protein homodimerization activity; receptor binding

Biological Process: glutathione metabolic process; linoleic acid metabolic process; nitrobenzene metabolic process; regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; xenobiotic catabolic process