Western Blot (WB)
(Western Blot Analysis of GroEL: Lane 1: MW Marker, Lane 2: GroEL (E Coli), (recombinant), Lane 3: HSP60 (human), (recombinant), Lane 4: HeLa (Heat Shocked), Cell Lysate, Lane 5: E Coli Cell Lysate all probed with GroEL (E Coli), pAb .)
Rabbit anti-E Coli GroEL Polyclonal Antibody | anti-Chaperonin 60 antibody
GroEL (E Coli) polyclonal antibody
Gene Names
groL; ECK4137; groEL; JW4103; mopA
Reactivity
E Coli
Applications
Western Blot
Purity
Protein A affinity purified.
Synonyms
GroEL; Polyclonal Antibody; GroEL (E Coli) polyclonal antibody; anti-Chaperonin 60 antibody
Host
Rabbit
Reactivity
E Coli
Clonality
Polyclonal
Specificity
Recognizes bacteria GroEL. Detects a band of ~60kDa by Western blot.
Purity/Purification
Protein A affinity purified.
Form/Format
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Sequence Length
548
Applicable Applications for anti-Chaperonin 60 antibody
Western Blot (WB)
Application Notes
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~60kDa by Western blot.
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~60kDa by Western blot.
Immunogen
E Coli GroEL.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Long term storage: -20 degree C
Avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
Western Blot (WB)
(Western Blot Analysis of GroEL: Lane 1: MW Marker, Lane 2: GroEL (E Coli), (recombinant), Lane 3: HSP60 (human), (recombinant), Lane 4: HeLa (Heat Shocked), Cell Lysate, Lane 5: E Coli Cell Lysate all probed with GroEL (E Coli), pAb .)
Western Blot (WB)
(Western Blot Analysis of GroEL: Lane 1: MW Marker, Lane 2: GroEL (E Coli), (recombinant), Lane 3: HSP60 (human), (recombinant), Lane 4: HeLa (Heat Shocked), Cell Lysate, Lane 5: E Coli Cell Lysate all probed with GroEL (E Coli), pAb .)
Related Product Information for anti-Chaperonin 60 antibody
GroEL and GroES are E Coli chaperonins, which are homologs of mitochondrial chaperonins Hsp60 and Hsp10. GroEL is a double toriodal assembly of 14 identical subunits which form two heptameric rings stacked back-to-back, with a cavity at each end. GroEL and its co-chaperonin GroES facilitate protein folding with an ATP-dependent mechanism.
Product Categories/Family for anti-Chaperonin 60 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
57,329 Da
NCBI Official Full Name
60 kDa chaperonin
NCBI Official Symbol
groL
NCBI Official Synonym Symbols
ECK4137; groEL; JW4103; mopA
NCBI Protein Information
Cpn60 chaperonin GroEL, large subunit of GroESL
UniProt Protein Name
60 kDa chaperonin
UniProt Gene Name
groL
UniProt Entry Name
CH60_ECOLI
NCBI Description
groL is an essential gene. [More information is available at EcoGene: EG10599]. The Escherichia coli chaperone protein GroEL (Hsp60) and its regulator GroES are necessary for the proper folding of certain proteins , . [More information is available at EcoCyc: EG10599].
Uniprot Description
Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.
Research Articles on Chaperonin 60
Similar Products
Product Notes
The Chaperonin 60 grol (Catalog #AAA565246) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GroEL (E Coli) polyclonal antibody reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's GroEL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Detects a band of ~60kDa by Western blot. Researchers should empirically determine the suitability of the Chaperonin 60 grol for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GroEL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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