Growth Factor Receptor Bound Protein 14 (Grb14) Recombinant Protein | Grb14 recombinant protein

Recombinant Growth Factor Receptor Bound Protein 14 (Grb14)

Cat. Num. AAA2086920

• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Growth Factor Receptor Bound Protein 14 (Grb14); Recombinant Growth Factor Receptor Bound Protein 14 (Grb14); GRB14 adapter protein; Grb14 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 453

• Applicable Applications for Grb14 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Fragment: Ala300~Leu540
• Tag: N-terminal His-tag
• Buffer Formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Source Note: Prokaryotic expression
• Organism Species: Human
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 9
• Subcellular Location: Cytoplasm. Golgi Apparatus Membrane. Peripheral Membrane Protein. Endosome Membrane.
• Usage: Reconstitute in sterile ddH2O.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Product Categories/Family for Grb14 recombinant protein

Recombinant Protein

NCBI and Uniprot Product Information

• NCBI GI #: 742068549
• NCBI GeneID: 2888
• NCBI Accession #: NP_001290351.1
• NCBI GenBank Nucleotide #: NM_001303422.1
• UniProt Accession #: Q14449; Q7Z6I1; B7Z7F9
• Molecular Weight: 30.9kDa
• NCBI Official Full Name: growth factor receptor-bound protein 14 isoform 2
• NCBI Official Synonym Full Names: growth factor receptor bound protein 14
• NCBI Official Symbol: GRB14
• NCBI Protein Information: growth factor receptor-bound protein 14
• UniProt Protein Name: Growth factor receptor-bound protein 14
• Protein Family: Growth factor receptor-bound protein
• UniProt Gene Name: GRB14

NCBI Description

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

GRB14: Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal. Belongs to the GRB7/10/14 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2q24.3

Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; plasma membrane

Biological Process: leukocyte migration