Glutathione Peroxidase 1 (GPX1) Recombinant Protein | GPX1 recombinant protein

Recombinant Glutathione Peroxidase 1 (GPX1)

Cat. Num. AAA2124436

• Gene Names: Gpx1; GSHPx; GSHPx-1
• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: Glutathione Peroxidase 1 (GPX1); Recombinant Glutathione Peroxidase 1 (GPX1); Glutathione Peroxidase 1; GSHPX1; Cellular glutathione peroxidase; GPX1 recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence: Gly48~Pro201

• Applicable Applications for GPX1 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Rat
• Tag: N-terminal His Tag
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 6.2
• Subcellular Location: Cytoplasm
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

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SDS-Page

(Figure. SDS-PAGE)

Product Categories/Family for GPX1 recombinant protein

Enzyme & Kinase; Metabolic pathway; Recombinant proteins/RP

NCBI and Uniprot Product Information

• NCBI GI #: 145275165
• NCBI GeneID: 24404
• NCBI Accession #: NP_110453.3
• NCBI GenBank Nucleotide #: NM_030826.4
• UniProt Accession #: P04041; O08946; Q4PIY2; Q91WZ5
• Molecular Weight: 22,305 Da
• NCBI Official Full Name: glutathione peroxidase 1
• NCBI Official Synonym Full Names: glutathione peroxidase 1
• NCBI Official Symbol: Gpx1
• NCBI Official Synonym Symbols: GSHPx; GSHPx-1
• NCBI Protein Information: glutathione peroxidase 1
• UniProt Protein Name: Glutathione peroxidase 1
• Protein Family: Hydroperoxy fatty acid reductase
• UniProt Gene Name: Gpx1
• UniProt Synonym Gene Names: GPx-1; GSHPx-1

NCBI Description

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Other studies indicate that H2O2 is also essential for growth-factor mediated signal transduction, mitochondrial function, and maintenance of thiol redox-balance; therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is the most abundant, is ubiquitously expressed and localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. It is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Jul 2016]

Uniprot Description

Protects the hemoglobin in erythrocytes from oxidative breakdown.