Typical Testing Data/Standard Curve (for reference only)
Monkey Glutamic Pyruvic Transaminase ELISA Kit | GPI/ALT elisa kit
Monkey Glutamic Pyruvic Transaminase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: GPT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPT antibody and an GPT-HRP conjugate. The assay sample and buffer are incubated together with GPT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPT concentration since GPT from samples and GPT-HRP conjugate compete for the anti-GPT antibody binding site. Since the number of sites is limited, as more sites are occupied by GPT from the sample, fewer sites are left to bind GPT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPT concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]
Uniprot Description
GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
Protein type: Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.2
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: cytosol
Molecular Function: alanine transaminase activity; pyridoxal phosphate binding
Biological Process: amino acid biosynthetic process; L-alanine catabolic process
Research Articles on GPI/ALT
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Product Notes
The Monkey GPI/ALT gpt (Catalog #AAA734823) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA734823 ELISA Kit recognizes Monkey GPI/ALT. It is sometimes possible for the material contained within the vial of "Glutamic Pyruvic Transaminase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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