Goat Glutamic Pyruvic Transaminase ELISA Kit | GPT elisa kit

Goat Glutamic Pyruvic Transaminase ELISA Kit

Cat. Num. AAA737268

• Gene Names: GPT; AAT1; ALT1; GPT1
• Reactivity: Goat
• Synonyms: Glutamic Pyruvic Transaminase; Goat Glutamic Pyruvic Transaminase ELISA Kit; GPT elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This GPT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Gaot GPT. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPT elisa kit

Principle of the Assay: GPT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPT antibody and an GPT-HRP conjugate. The assay sample and buffer are incubated together with GPT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPT concentration since GPT from samples and GPT-HRP conjugate compete for the anti-GPT antibody binding site. Since the number of sites is limited, as more sites are occupied by GPT from the sample, fewer sites are left to bind GPT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPT concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885351
• NCBI GeneID: 2875
• NCBI Accession #: NP_005300.1
• NCBI GenBank Nucleotide #: NM_005309.2
• Molecular Weight: 54,637 Da
• NCBI Official Full Name: alanine aminotransferase 1
• NCBI Official Synonym Full Names: glutamic-pyruvate transaminase (alanine aminotransferase)
• NCBI Official Symbol: GPT
• NCBI Official Synonym Symbols: AAT1; ALT1; GPT1
• NCBI Protein Information: alanine aminotransferase 1; GPT 1; glutamic-alanine transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1
• UniProt Protein Name: Alanine aminotransferase 1
• Protein Family: Alanine aminotransferase
• UniProt Gene Name: GPT
• UniProt Synonym Gene Names: AAT1; GPT1; ALT1; GPT 1
• UniProt Entry Name: ALAT1_HUMAN

NCBI Description

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

Uniprot Description

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Protein type: EC 2.6.1.2; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function: alanine transaminase activity; pyridoxal phosphate binding

Biological Process: amino acid biosynthetic process; L-alanine catabolic process