GPR37 recombinant protein

GPR37 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121432

• Gene Names: GPR37; PAELR; EDNRBL; hET(B)R-LP
• Purity: >80% as determined by SDS-PAGE
• Synonyms: GPR37; GPR37 Protein; Human; Recombinant (His Tag); Human GPR37 Protein (His Tag); EDNRBL Protein; hET(B)R-LP Protein; PAELR Protein; G protein-coupled receptor 37 (endothelin receptor type B-like); GPR37 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Met265

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 27
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human GPR37 (NP_005293.1) the first extracellular domaqin (Met 1-Met 265) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GPR37 recombinant protein

Background: GPR37 (cathepsin Z) is an orphan receptor that belongs to the G-protein coupled receptor 1 family. G protein-coupled receptors are a large protein family comprised of transmembrane receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. They only exist in eukaryotes, including yeast, choanoflagellates, and animals. These receptors are bonded and activated by light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters. These ligands vary in size from small molecules to peptides to large proteins. G protein-coupled receptors are involved in many diseases and are also the target of approximately 40% of all modern medicinal drugs. GPR37 is expressed in the brain and spinal cord, and at lower levels in the testis, placenta, and liver, but no detectable expression is observed in any other tissue. GPR37 may have a unique functional role in the central nervous system.

References

Marazziti D, et al. (1997) Cloning of GPR37, a gene located on chromosome 7 encoding a putative G-protein-coupled peptide receptor, from a human frontal brain EST library. Genomics. 45(1):68-77.Imai, et al. (2002) CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity. Mol Cell. 10(1):55-67.Imai, et al. (2001) An unfolded putative transmembrane polypeptide, which can lead to endoplasmic reticulum stress, is a substrate of Parkin. Cell. 105(7):891-902.

NCBI and Uniprot Product Information

• NCBI GI #: 4885323
• NCBI GeneID: 2861
• NCBI Accession #: NP_005293.1
• NCBI GenBank Nucleotide #: NM_005302.3
• UniProt Accession #: O15354; O00348; O14768; Q8TD39
• Molecular Weight: 67,114 Da
• NCBI Official Full Name: probable G-protein coupled receptor 37
• NCBI Official Synonym Full Names: G protein-coupled receptor 37 (endothelin receptor type B-like)
• NCBI Official Symbol: GPR37
• NCBI Official Synonym Symbols: PAELR; EDNRBL; hET(B)R-LP
• NCBI Protein Information: probable G-protein coupled receptor 37; ETBR-LP-1; endothelin B receptor-like protein 1; Parkin-associated endothelin receptor-like receptor
• UniProt Protein Name: Probable G-protein coupled receptor 37
• Protein Family: Prosaposin receptor
• UniProt Gene Name: GPR37
• UniProt Synonym Gene Names: ETBR-LP-1; PAELR
• UniProt Entry Name: GPR37_HUMAN

NCBI Description

This gene is a member of the G protein-coupled receptor family. The encoded protein contains seven transmembrane domains and is found in cell and endoplasmic reticulum membranes. G protein-coupled receptors are involved in translating outside signals into G protein mediated intracellular effects. This gene product interacts with Parkin and is involved in juvenile Parkinson disease. [provided by RefSeq, Oct 2012]

Uniprot Description

GPR37: Orphan receptor. May have a unique functional role in the central nervous system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; ubiquitin ligase complex; receptor complex

Molecular Function: G-protein coupled receptor activity; protein binding; ubiquitin protein ligase binding; heat shock protein binding; peptide receptor activity, G-protein coupled; Hsp70 protein binding; peptide binding

Biological Process: positive regulation of dopamine metabolic process; G-protein coupled receptor protein signaling pathway; positive regulation of MAPKKK cascade; G-protein signaling, adenylate cyclase inhibiting pathway; dopamine biosynthetic process; locomotion during locomotory behavior