Western Blot (WB)
(IKKb mAb (Clone 10A9B6) tested by Western blot. Immunoprecipitation of IKKb. 2 ug of IKKb mAb (Clone 10A9B6) was used to immunoprecipitate IKKb from 1 x 106 cells. The immunoprecipitated protein was detected by Western blotting using the same antibody. The IKKb protein detected was ~87 kDa in size.)
Mouse anti-Human IKKb Monoclonal Antibody | anti-IKKb antibody
IKKb antibody (mAb)(Clone 10A9B6)
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Affinity Purified
Synonyms
IKKb; Monoclonal Antibody; IKKb antibody (mAb)(Clone 10A9B6); IKBKB; IKK2; IMD15; NFKBIKB; IKK-beta; anti-IKKb antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10A9B6
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Concentration
0.5ug/ul (varies by lot)
Applicable Applications for anti-IKKb antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
IP: 2ug per IP
WB: 1-2ug/ml dilution
IP: 2ug per IP
WB: 1-2ug/ml dilution
Immunogen
This IKKb antibody was raised against a 6xHis-Tag full-length protein corresponding to human IKKb.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(IKKb mAb (Clone 10A9B6) tested by Western blot. Immunoprecipitation of IKKb. 2 ug of IKKb mAb (Clone 10A9B6) was used to immunoprecipitate IKKb from 1 x 106 cells. The immunoprecipitated protein was detected by Western blotting using the same antibody. The IKKb protein detected was ~87 kDa in size.)
Western Blot (WB)
(IKKb mAb (Clone 10A9B6) tested by Western blot. Immunoprecipitation of IKKb. 2 ug of IKKb mAb (Clone 10A9B6) was used to immunoprecipitate IKKb from 1 x 106 cells. The immunoprecipitated protein was detected by Western blotting using the same antibody. The IKKb protein detected was ~87 kDa in size.)
Related Product Information for anti-IKKb antibody
Short Description: Our IKKb antibody (mAb)(Clone 10A9B6) was raised in a Mouse host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: IKKb (IkB Kinase beta subunit)-The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB (NFkB p50 & NFkB p65). The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IkBa at Ser32 and Ser36. This results ultimately in the degradation of IkB and the activation and nuclear translocation of NFkB. While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK beta appears to be the principal kinase, as IKKa is not required for activation of the IKK complex and the subsequent degradation of IkBa. Once activated, IKKb autophosphorylates, which decreases IKK activity, thereby preventing a prolonged activation of inflammation.
Background: IKKb (IkB Kinase beta subunit)-The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB (NFkB p50 & NFkB p65). The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IkBa at Ser32 and Ser36. This results ultimately in the degradation of IkB and the activation and nuclear translocation of NFkB. While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK beta appears to be the principal kinase, as IKKa is not required for activation of the IKK complex and the subsequent degradation of IkBa. Once activated, IKKb autophosphorylates, which decreases IKK activity, thereby preventing a prolonged activation of inflammation.
Product Categories/Family for anti-IKKb antibody
NCBI and Uniprot Product Information
Molecular Weight
87kDa
Similar Products
Product Notes
The IKKb (Catalog #AAA388671) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IKKb antibody (mAb)(Clone 10A9B6) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IKKb can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 2ug per IP WB: 1-2ug/ml dilution. Researchers should empirically determine the suitability of the IKKb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IKKb, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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