IgG (H&L) Secondary Antibody | IgG secondary antibody
Alkaline Phosphatase Conjugated Affinity Purified anti-Swine IgG (H&L) [Goat]
Reactivity
Swine
Applications
Immunoblot, Western Blot, Western Blot, Dot Blot, ELISA, Immunohistochemistry
Synonyms
IgG (H&L); Alkaline Phosphatase Conjugated Affinity Purified anti-Swine IgG (H&L) [Goat]; IgG secondary antibody
Host
Goat
Reactivity
Swine
Concentration
1.0 mg/ml (by UV absorbance at 280 nm) (varies by lot)
Applicable Applications for IgG secondary antibody
Immunoblotting, Western Blot (WB), Dot Blot (DB), ELISA (EIA), Immunohistochemistry (IHC), Other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency
Application Notes
ELISA: 1:2,000 - 1:10,000
WB: 1:500 - 1:2,500
IHC: 1:200 - 1:1,000
WB: 1:500 - 1:2,500
IHC: 1:200 - 1:1,000
Immunogen
Swine IgG whole molecule
Storage Buffer
0.05M Tris Chloride,0.15M Sodium Chloride,0.001M Magnesium Chloride,0.0001M Zinc Chloride,50% (v/v) Glycerol; pH 8.0
Stabilizer
10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free
Preparation and Storage
Store vial at 4 degree C before opening. DO NOT FREEZE. This product is stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Related Product Information for IgG secondary antibody
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Swine IgG and Swine Serum.
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Product Notes
The IgG (Catalog #AAA417849) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Alkaline Phosphatase Conjugated Affinity Purified anti-Swine IgG (H&L) [Goat] reacts with Swine and may cross-react with other species as described in the data sheet. AAA Biotech's IgG (H&L) can be used in a range of immunoassay formats including, but not limited to, Immunoblotting, Western Blot (WB), Dot Blot (DB), ELISA (EIA), Immunohistochemistry (IHC), Other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. ELISA: 1:2,000 - 1:10,000 WB: 1:500 - 1:2,500 IHC: 1:200 - 1:1,000. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG (H&L), Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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