Rat Gasdermin D (GSDMD) ELISA Kit | GSDMD elisa kit

Rat Gasdermin D (GSDMD) ELISA Kit

Cat. Num. AAA2705517

• Gene Names: GSDMD; DF5L; DFNA5L; FKSG10; GSDMDC1
• Reactivity: Rat
• Synonyms: Gasdermin D (GSDMD); Rat Gasdermin D (GSDMD) ELISA Kit; GSDMDC1; DF5L; Gasdermin Domain Containing 1; GSDMD elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Gasdermin D (GSDMD).; No significant cross-reactivity or interference between Gasdermin D (GSDMD) and analogues was observed.
• Sequence Length: 484

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gasdermin D (GSDMD) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gasdermin D (GSDMD) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GSDMD elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gasdermin D (GSDMD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gasdermin D (GSDMD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gasdermin D (GSDMD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gasdermin D (GSDMD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 261878494
• NCBI GeneID: 79792
• NCBI Accession #: NP_001159709.1
• NCBI GenBank Nucleotide #: NM_001166237.1
• UniProt Accession #: P57764; Q96Q98; A8K702; D3DWJ9
• Molecular Weight: 52,801 Da
• NCBI Official Full Name: gasdermin-D
• NCBI Official Synonym Full Names: gasdermin D
• NCBI Official Symbol: GSDMD
• NCBI Official Synonym Symbols: DF5L; DFNA5L; FKSG10; GSDMDC1
• NCBI Protein Information: gasdermin-D
• UniProt Protein Name: Gasdermin-D
• Protein Family: Gasdermin
• UniProt Gene Name: GSDMD
• UniProt Synonym Gene Names: DFNA5L; GSDMDC1; GSDMD-CT

NCBI Description

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Uniprot Description

FKSG10: Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol; extracellular region; extracellular space; ficolin-1-rich granule lumen; NLRP3 inflammasome complex; nucleoplasm; plasma membrane; tertiary granule lumen

Molecular Function: cardiolipin binding; phosphatidic acid binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-4-phosphate binding; phosphatidylserine binding; protein binding

Biological Process: cellular response to extracellular stimulus; cytolysis; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; inflammatory response; innate immune response; neutrophil degranulation; pore complex assembly; positive regulation of interleukin-1 beta secretion; protein homooligomerization; pyroptosis