Human vinculin ELISA Kit | VCL elisa kit

Human Vinculin, VCL ELISA Kit

Cat. Num. AAA909803

• Gene Names: VCL; MV; MVCL; CMD1W; CMH15; HEL114
• Reactivity: Human
• Synonyms: vinculin; Human Vinculin; VCL ELISA Kit; Human Vinculin (VCL) ELISA kit; RP11-178G16.3; CMD1W; MVCL; metavinculin; VCL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human VCL. No significant cross-reactivity or interference between human VCL and analogues was observed.
• Sequence Length: 1066

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 28 pg/ml -1800 pg/ml.
• Sensitivity: The minimum detectable dose of human VCL is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VCL elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VCL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCL bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4507877
• NCBI GeneID: 7414
• NCBI Accession #: NP_003364.1
• NCBI GenBank Nucleotide #: NM_003373.3
• UniProt Accession #: P18206; Q16450; Q5SWX2; Q7Z3B8; Q8IXU7
• Molecular Weight: 123,799 Da
• NCBI Official Full Name: vinculin isoform VCL
• NCBI Official Synonym Full Names: vinculin
• NCBI Official Symbol: VCL
• NCBI Official Synonym Symbols: MV; MVCL; CMD1W; CMH15; HEL114
• NCBI Protein Information: vinculin; metavinculin; epididymis luminal protein 114
• UniProt Protein Name: Vinculin
• Protein Family: Vinculin
• UniProt Gene Name: VCL
• UniProt Synonym Gene Names: MV
• UniProt Entry Name: VINC_HUMAN

NCBI Description

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: protein complex; focal adhesion; costamere; extracellular region; fascia adherens; intercellular junction; cytosol; cell-matrix junction; actin cytoskeleton; adherens junction; cell-cell adherens junction; cytoskeleton; plasma membrane; vesicle

Molecular Function: protein binding; cadherin binding; beta-catenin binding; actin binding; structural molecule activity; alpha-catenin binding

Biological Process: lamellipodium biogenesis; platelet activation; apical junction assembly; platelet degranulation; muscle contraction; axon extension; cell-matrix adhesion; morphogenesis of an epithelium; cell motility; cell adhesion; blood coagulation; negative regulation of cell migration

Disease: Cardiomyopathy, Dilated, 1w