Fish Progonadoliberin-1 ELISA Kit | GNRH1 elisa kit

Fish Progonadoliberin-1 ELISA Kit

Cat. Num. AAA288500

• Gene Names: GNRH1; GRH; GNRH; LHRH; LNRH
• Reactivity: Fish
• Synonyms: Progonadoliberin-1; Fish Progonadoliberin-1 ELISA Kit; GNRH1 elisa kit

• Reactivity: Fish
• Specificity: This assay recognizes recombinant and natural Fish Progonadoliberin-1. No significant cross-reactivity or interference was observed.
• Sequence Length: 92

• Samples: Serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
• Assay Type: Competitive
• Detection Range: 78.0-5000 pg/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of fish Progonadoliberin-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GNRH1 elisa kit

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Progonadoliberin-1, During the reaction, Progonadoliberin-1 in the sample or standard competes with a fixed amount of biotin-labeled Progonadoliberin-1 for sites on a pre-coated Monoclonal antibody specific to Progonadoliberin-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Progonadoliberin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 133908612
• NCBI GeneID: 2796
• NCBI Accession #: NP_001076580.1
• NCBI GenBank Nucleotide #: NM_001083111.1
• Molecular Weight: 10,380 Da
• NCBI Official Full Name: progonadoliberin-1 isoform 2 preproprotein
• NCBI Official Synonym Full Names: gonadotropin releasing hormone 1
• NCBI Official Symbol: GNRH1
• NCBI Official Synonym Symbols: GRH; GNRH; LHRH; LNRH
• NCBI Protein Information: progonadoliberin-1
• UniProt Protein Name: Progonadoliberin-1
• Protein Family: Progonadoliberin
• UniProt Gene Name: GNRH1
• UniProt Synonym Gene Names: GNRH; GRH; LHRH; GnRH-I; LH-RH I
• UniProt Entry Name: GON1_HUMAN

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate a peptide that is a member of the gonadotropin-releasing hormone (GnRH) family of peptides. Alternative splicing results in multiple transcript variants, at least one of which is secreted and then cleaved to generate gonadoliberin-1 and GnRH-associated peptide 1. Gonadoliberin-1 stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. Mutations in this gene are associated with hypogonadotropic hypogonadism. [provided by RefSeq, Nov 2015]

Uniprot Description

GNRH1: Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones. Belongs to the GnRH family.

Protein type: Hormone; Secreted; Secreted, signal peptide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8p21-p11.2

Cellular Component: dendrite; extracellular region; extracellular space; Golgi-associated vesicle; mitochondrion; nerve terminal; perikaryon

Molecular Function: hormone activity; luteinizing hormone-releasing factor activity

Biological Process: aging; cell-cell signaling; female pregnancy; male sex determination; multicellular organismal development; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of immature T cell proliferation; regulation of gene expression; response to corticosteroid stimulus; response to ethanol; response to lipopolysaccharide; response to testosterone stimulus; signal transduction

Disease: Hypogonadotropic Hypogonadism 7 With Or Without Anosmia