Activity
(Sample Cell Assay Data: ED50 not determined.)
GMFB recombinant protein
GMFB
Applications
Cell Assay
Synonyms
GMFB; GMF-beta; Recombinant Human Glia Maturation Factor Beta (GMFB); GMFB recombinant protein
Host
E Coli
Form/Format
Recombinant GMFB was lyophilized from a 0.2 um filtered 20mM PB,130mM NaCl solution pH7.5.
Applicable Applications for GMFB recombinant protein
Cell Assay
Species
Human
Endotoxin Level
<1.0EU/ug of recombinant protein as determined by the LAL method
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Activity
(Sample Cell Assay Data: ED50 not determined.)
Activity
(Sample Cell Assay Data: ED50 not determined.)
SDS-Page
(Purity: Recombinant Human GMFB resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)
SDS-Page
(Purity: Recombinant Human GMFB resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)
Related Product Information for GMFB recombinant protein
Recombinant Human GMFB was expressed in E Coli cells. The protein accession number is P60983
GMF-beta is a 17-kDa brain-specific protein that was isolated from bovine brain homogenate as a substance inducing the maturation of normal neurons as well as glial cells, and at first, it was considered to be a neurotrophic factor. The amino acid sequence of GMFB is highly conserved among many species, suggesting that it plays basic roles across many species. The expression of GMF-b is largely limited to the brain, especially the glial cells and some neurons. Schwann cells of the distal segment of the transected nerve express GMF-b, and this induction of GMF-b coincides with the temporal expression of nerve growth factor receptors in the cell.
GMF-beta is a 17-kDa brain-specific protein that was isolated from bovine brain homogenate as a substance inducing the maturation of normal neurons as well as glial cells, and at first, it was considered to be a neurotrophic factor. The amino acid sequence of GMFB is highly conserved among many species, suggesting that it plays basic roles across many species. The expression of GMF-b is largely limited to the brain, especially the glial cells and some neurons. Schwann cells of the distal segment of the transected nerve express GMF-b, and this induction of GMF-b coincides with the temporal expression of nerve growth factor receptors in the cell.
Product Categories/Family for GMFB recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
16,713 Da
NCBI Official Full Name
GMFB
UniProt Protein Name
Glia maturation factor beta
Protein Family
UniProt Gene Name
GMFB
UniProt Synonym Gene Names
GMF-beta
UniProt Entry Name
GMFB_HUMAN
Uniprot Description
GMF-beta: This protein causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells. Belongs to the actin-binding proteins ADF family. GMF subfamily.
Protein type: Actin-binding; Inhibitor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 14q22.2
Cellular Component: intracellular
Molecular Function: signal transducer activity; growth factor activity; protein kinase inhibitor activity; enzyme activator activity; actin binding
Biological Process: positive regulation of catalytic activity; nervous system development; negative regulation of protein kinase activity; locomotory behavior; learning; signal transduction; protein amino acid phosphorylation
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Product Notes
The GMFB gmfb (Catalog #AAA517035) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GMFB can be used in a range of immunoassay formats including, but not limited to, Cell Assay. Researchers should empirically determine the suitability of the GMFB gmfb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GMFB, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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