Glucose Regulated Protein 94, Canine Recombinant Protein
Glucose Regulated Protein 94, Recombinant, Canine (Grp 94, gp96)
Applications
Western Blot
Purity
Highly Purified90% as determined by SDS-PAGE analysis.
Synonyms
Glucose Regulated Protein 94; Canine; Recombinant; Canine (Grp 94; gp96); Canine recombinant protein
Purity/Purification
Highly Purified
90% as determined by SDS-PAGE analysis.
90% as determined by SDS-PAGE analysis.
Form/Format
Supplied as a liquid in 20mM MOPS, pH 7.5, 0.5mM EDTA.
Applicable Applications for Glucose Regulated Protein 94, Canine recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 100ng of protein per lane should be sufficient when used in a 15-well minigel colorimetric system. The material should be diluted in an SDS containing sample buffer and heated at 100 degree C for 5 minutes prior to use.
Dilution: Western Blot: 100ng of protein per lane should be sufficient when used in a 15-well minigel colorimetric system. The material should be diluted in an SDS containing sample buffer and heated at 100 degree C for 5 minutes prior to use.
Preparation and Storage
-70 degree C
Related Product Information for Glucose Regulated Protein 94, Canine recombinant protein
Glucose-regulated protein, Grp94 or gp96, is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belong to the Hsp90 family of molecular chaperones. Grp94 and other resident soluble proteins of the ER such as members of the Ca(2+) binding protein subfamily (CaBP), CaBPI and CaBP2 as well as calreticulin possesses the COOH-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL) which is a sorting signal that is thought to lead to the retention of these proteins in the pre-Golgi compartments (1). Grp94 expression is upregulated by stress conditions such as glucose starvation and heat shock, which promote protein misfolding or unfolding (2). In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells (3,4). Grp94 and Hsp90 share high sequence identity and presumably identical adenosine nucleotide-dependent modes of regulation, but earlier data suggests that Hsp90 and Grp94 may differ in their nucleotide binding properties. The N-terminal domain of eukaryotic Hsp90 proteins contains a conserved adenosine nucleotide binding pocket which also serves as the binding site for the Hsp90 inhibitors geldanamycin and radicicol, however, the molecular basis for adenosine nucleotide-dependent regulation of Grp94 remains to be established. Recent data has identified a ligand dependent regulation of Grp94 function and suggest a model whereby Grp94 function is regulated through a ligand-dependent conversion of Grp94 from an inactive to an active conformation (5, 6).
Product Categories/Family for Glucose Regulated Protein 94, Canine recombinant protein
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Product Notes
The Glucose Regulated Protein 94, Canine (Catalog #AAA636235) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Glucose Regulated Protein 94, Canine can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 100ng of protein per lane should be sufficient when used in a 15-well minigel colorimetric system. The material should be diluted in an SDS containing sample buffer and heated at 100 degree C for 5 minutes prior to use. Researchers should empirically determine the suitability of the Glucose Regulated Protein 94, Canine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glucose Regulated Protein 94, Canine, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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