GRP94 Recombinant Protein | HSP90B1 recombinant protein

GRP94, Recombinant, Human (heat shock protein 90kD beta, Grp94, member 1, 94 kD glucose-regulated protein, ECGP, HSP90B1, Endoplasmin precursor, GP96, gp96 homolog, GRP94, Heat shock protein 90kD beta member 1, TRA1, Tumor Rejection Antigen 1)

Cat. Num. AAA637299

• Gene Names: HSP90B1; TRA1; GRP94
• Purity: Highly Purified; 90% (SDS-PAGE)
• Synonyms: GRP94; Recombinant; Human (heat shock protein 90kD beta; Grp94; member 1; 94 kD glucose-regulated protein; ECGP; HSP90B1; Endoplasmin precursor; GP96; gp96 homolog; Heat shock protein 90kD beta member 1; TRA1; Tumor Rejection Antigen 1); HSP90B1 recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; 90% (SDS-PAGE)
• Form/Format: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 1mM EDTA, 0.1M sodium chloride, 10% glycerol.
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMDDE VDVDGTVEED LGKSREGSRT DDEVVQREEE AIQLDGLNAS QIRELREKSE KFAFQAEVNR; MMKLIINSLY KNKEIFLREL ISNASDALDK IRLISLTDEN ALSGNEELTV KIKCDKEKNL LHVTDTGVGM TREELVKNLG TIAKSGTSEF LNKMTEAQED; GQSTSELIGQ FGVGFYSAFL VADKVIVTSK HNNDTQHIWE SDSNEFSVIA DPRGNTLGRG TTITLVLKEE ASDYLELDTI KNLVKKYSQF INFPIYVWSS; KTETVEEPME EEEAAKEEKE ESDDEAAVEE EEEEKKPKTK KVEKTVWDWE LMNDIKPIWQ RPSKEVEEDE YKAFYKSFSK ESDDPMAYIH FTAEGEVTFK; SILFVPTSAP RGLFDEYGSK KSDYIKLYVR RVFITDDFHD MMPKYLNFVK GVVDSDDLPL NVSRETLQQH KLLKVIRKKL VRKTLDMIKK IADDKYNDTF; WKEFGTNIKL GVIEDHSNRT RLAKLLRFQS SHHPTDITSL DQYVERMKEK QDKIYFMAGS SRKEAESSPF VERLLKKGYE VIYLTEPVDE YCIQALPEFD; GKRFQNVAKE GVKFDESEKT KESREAVEKE FEPLLNWMKD KALKDKIEKA VVSQRLTESP CALVASQYGW SGNMERIMKA QAYQTGKDIS TNYYASQKKT; FEINPRHPLI RDMLRRIKED EDDKTVLDLA VVLFETATLR SGYLLPDTKA YGDRIERMLR LSLNIDPDAK VEEEPEEEPE ETAEDTTEDT EQDEDEEMDV; GTDEEEETAK ESTAEKDEL

• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(15% SDS-PAGE using 3ug of G3057-44C.)

Related Product Information for HSP90B1 recombinant protein

GRP94, also known as Heat shock protein 90kDa beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells. Recombinant human GRP94 protein, fused to His-tag at N-terminus.

Product Categories/Family for HSP90B1 recombinant protein

Molecular Biology; MB-Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 403497
• NCBI GeneID: 404019
• UniProt Accession #: P41148
• Molecular Weight: 94.4 kD
• NCBI Official Full Name: GRP94
• NCBI Official Symbol: HSP90B1
• NCBI Official Synonym Symbols: TRA1; GRP94
• NCBI Protein Information: endoplasmin; GRP-94; tumor rejection antigen 1; 94 kDa glucose-regulated protein; tumor rejection antigen (gp96) 1; heat shock protein 90 kDa beta member 1
• UniProt Protein Name: Endoplasmin
• Protein Family: Endoplasmin
• UniProt Gene Name: HSP90B1
• UniProt Synonym Gene Names: GRP94; TRA1; GRP-94
• UniProt Entry Name: ENPL_CANFA

Uniprot Description

GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family.

Protein type: Secreted; Apoptosis; Heat shock protein; Secreted, signal peptide

Cellular Component: extracellular matrix; endoplasmic reticulum membrane; focal adhesion; sarcoplasmic reticulum; endoplasmic reticulum lumen; perinuclear region of cytoplasm; plasma membrane; melanosome; midbody; nucleus; cytosol

Molecular Function: low-density lipoprotein receptor binding; RNA binding; unfolded protein binding; virion binding; protein phosphatase binding; ATP binding

Biological Process: actin rod formation; ER-associated protein catabolic process; protein folding; response to hypoxia; regulation of phosphoprotein phosphatase activity; negative regulation of apoptosis