Western Blot (WB)
(Western blot analysis of extracts of NCL-H460, using EDA antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit EDA Polyclonal Antibody | anti-EDA antibody
EDA Antibody
Gene Names
EDA; ED1; HED; EDA1; EDA2; HED1; ODT1; XHED; ECTD1; XLHED; ED1-A1; ED1-A2; EDA-A1; EDA-A2; TNLG7C; STHAGX1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
EDA; Polyclonal Antibody; EDA Antibody; ECTD1; Ectodermal dysplasia 1; anhidrotic; Ectodermal dysplasia protein; Ectodermal dysplasia; anhidrotic (hypohydrotic); Ectodysplasin A; membrane form; secreted form; ECTODYSPLASIN A1 ISOFORM; ECTODYSPLASIN A2 ISOFORM; ECTODYSPLASIN; Ectodysplasin-A; ED1 A1; ED1 A2; ED1; ED1 GENE; Eda A1; Eda A2; eda; EDA protein; EDA protein homolog; EDA_HUMAN; EDA1; EDA1 GENE; EDA2; HED; HED1; ODT1; Oligodontia 1; STHAGX1; Ta; Tabby; Tabby protein; X linked anhidroitic ectodermal dysplasia protein; XHED; XLHED; anti-EDA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
EDA antibody detects endogenous levels of total EDA
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
389
Applicable Applications for anti-EDA antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthetic peptide of human EDA
Subcellular Location
Secreted and Cell Membrane.
Tissue Specificity
Not abundant; expressed in specific cell types of ectodermal (but not mesodermal) origin of keratinocytes, hair follicles, sweat glands. Also in adult heart, liver, muscle, pancreas, prostate, fetal liver, uterus, small intestine and umbilical chord.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit
Similarity
Pig (91%), Bovine (91%), Horse (91%), Sheep (91%), Rabbit (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of NCL-H460, using EDA antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts of NCL-H460, using EDA antibody. The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-EDA antibody
Description: The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene.
Function: Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R (PubMed:8696334, PubMed:11039935, PubMed:27144394). May also play a role in cell adhesion (By similarity).
Subunit Structure: Homotrimer. The homotrimers may then dimerize and form higher-order oligomers.
Post-translational Modifications: N-glycosylated. Processing by furin produces a secreted form.
Similarity: Belongs to the tumor necrosis factor family.
Function: Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R (PubMed:8696334, PubMed:11039935, PubMed:27144394). May also play a role in cell adhesion (By similarity).
Subunit Structure: Homotrimer. The homotrimers may then dimerize and form higher-order oligomers.
Post-translational Modifications: N-glycosylated. Processing by furin produces a secreted form.
Similarity: Belongs to the tumor necrosis factor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 49 kDa
Predicted: 42 kDa
Predicted: 42 kDa
NCBI Official Full Name
ectodysplasin-A isoform 2
NCBI Official Synonym Full Names
ectodysplasin A
NCBI Official Symbol
EDA
NCBI Official Synonym Symbols
ED1; HED; EDA1; EDA2; HED1; ODT1; XHED; ECTD1; XLHED; ED1-A1; ED1-A2; EDA-A1; EDA-A2; TNLG7C; STHAGX1
NCBI Protein Information
ectodysplasin-A
UniProt Protein Name
Ectodysplasin-A
Protein Family
UniProt Gene Name
EDA
UniProt Synonym Gene Names
ED1; EDA2; EDA protein
NCBI Description
The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R (PubMed:8696334, PubMed:11039935, PubMed:27144394). May also play a role in cell adhesion ().
Research Articles on EDA
Similar Products
Product Notes
The EDA eda (Catalog #AAA9607755) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EDA Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EDA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the EDA eda for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EDA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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