SDS-Page
glpE recombinant protein
glpE, 1-108aa, E Coli, His tag, E Coli
Gene Names
glpE; ECK3411; JW3388
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
glpE; 1-108aa; E Coli; His tag; Thiosulfate:cyanide sulfurtransferase (rhodanese); ECK3411; JW3388; glpE recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDQFECI NVADAHQKLQ EKEAVLVDIR DPQSFAMGHA VQAFHLTNDT LGAFMRDNDF DTPVMVMCYH GNSSKGAAQY LLQQGYDVVY SIDGGFEAWQ RQFPAEVAYG A
Sequence Length
108
Applicable Applications for glpE recombinant protein
SDS-PAGE
Antigen Species
E Coli
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for glpE recombinant protein
Thiosulfate:cyanide sulfurtransferase (rhodanese), also known as glpE, is a mitochondrial matrix enzyme that is encoded by the nucleus. glpE catalyzes the sulfur-transfer reaction in which a sulfur atom is transferred from thiosulfate to cyanide by a double-displacement mechanism. Escherichia coli glpE is a prototype for the single-domain rhodanese superfamily. Recombinant E Coli glpE protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for glpE recombinant protein
References
Li H., et al. (2011) Biomol NMR Assign. 5 (1):97-9; Ray WK., et al. (2000) J Bacteriol. 182 (8):2277-84.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14.5kDa (131aa), confirmed by MALDI-TOF
NCBI Official Full Name
thiosulfate:cyanide sulfurtransferase (rhodanese)
NCBI Official Symbol
glpE
NCBI Official Synonym Symbols
ECK3411; JW3388
NCBI Protein Information
thiosulfate:cyanide sulfurtransferase (rhodanese)
UniProt Protein Name
Thiosulfate sulfurtransferase GlpE
Protein Family
UniProt Gene Name
glpE
NCBI Description
glpE, yceA and yibN are paralogs. [More information is available at EcoGene: EG10395].
Uniprot Description
Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide. The relatively low affinity of GlpE for both thiosulfate and cyanide suggests that these compounds are not the physiological substrates. Thioredoxin 1 or related dithiol proteins could instead be the physiological sulfur-acceptor substrate. Possible association with the metabolism of glycerol-phosphate remains to be elucidated.
Research Articles on glpE
Similar Products
Product Notes
The glpE glpe (Catalog #AAA205141) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's glpE can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the glpE glpe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMDQFECI NVADAHQKLQ EKEAVLVDIR DPQSFAMGHA VQAFHLTNDT LGAFMRDNDF DTPVMVMCYH GNSSKGAAQY LLQQGYDVVY SIDGGFEAWQ RQFPAEVAYG A. It is sometimes possible for the material contained within the vial of "glpE, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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