Noradrenaline (NE) DIY ELISA Kit | NE diy elisa kit

General Noradrenaline (NE) ELISA Kit DIY Materials

Cat. Num. AAA2088925

• Gene Names: SLC6A2; NET; NAT1; NET1; SLC6A5
• Reactivity: Pan-species (General)
• Synonyms: Noradrenaline (NE); General Noradrenaline (NE) ELISA Kit DIY Materials; NA; NAd; Norepinephrine; Norepi; NE diy elisa kit

• Reactivity: Pan-species (General)
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Noradrenaline (NE). No significant cross-reactivity or interference between Noradrenaline (NE) and analogues was observed.
• Sequence Length: 617

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 123.5-10000pg/mL
• Sensitivity: 49.2pg/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for NE diy elisa kit

Signal transduction; Infection immunity; Neuroscience; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 189258
• NCBI GeneID: 6530
• Molecular Weight: 57,370 Da
• NCBI Official Full Name: noradrenaline transporter
• NCBI Official Synonym Full Names: solute carrier family 6 member 2
• NCBI Official Symbol: SLC6A2
• NCBI Official Synonym Symbols: NET; NAT1; NET1; SLC6A5
• NCBI Protein Information: sodium-dependent noradrenaline transporter
• UniProt Protein Name: Sodium-dependent noradrenaline transporter
• UniProt Gene Name: SLC6A2
• UniProt Synonym Gene Names: NAT1; NET1; SLC6A5; NET

NCBI Description

This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

Uniprot Description

SLC6A2: Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A2 are a cause of orthostatic intolerance (OI). OI is a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. It is associated with postural tachycardia. Plasma norepinephrine concentration is abnormally high. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: membrane; neuron projection; plasma membrane

Molecular Function: dopamine:sodium symporter activity; monoamine transmembrane transporter activity; norephinephrine:sodium symporter activity; protein binding

Biological Process: dopamine uptake; monoamine transport; norepinephrine transport; synaptic transmission; transport

Disease: Orthostatic Intolerance