General Noradrenaline (NE) ELISA Kit | NE elisa kit

General Noradrenaline (NE) ELISA Kit

Cat. Num. AAA2700394

• Gene Names: SLC6A2; NET; NAT1; NET1; SLC6A5
• Reactivity: General
• Synonyms: Noradrenaline (NE); General Noradrenaline (NE) ELISA Kit; NA; NAd; Norepinephrine; Norepi; NE elisa kit

• Reactivity: General
• Specificity: This assay has high sensitivity and excellent specificity for detection of NMU. No significant cross-reactivity or interference between NMU and analogues was observed.
• Sequence Length: 617

• Samples: Mouse Serum, Plasma And Other Biological Fluids
• Assay Type: Quantitative Competitive
• Detection Range: 16-10,000pg/mL
• Sensitivity: 8pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NMU were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NMU were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NE elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NMU in mouse serum, plasma and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to NMU has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled NMU and unlabeled NMU (Standards or samples) with the pre-coated antibody specific to NMU. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NMU in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of NMU in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 189258
• NCBI GeneID: 6530
• UniProt Accession #: P23975; Q96KH8; B2R707; B4DX48
• Molecular Weight: 57,370 Da
• NCBI Official Full Name: noradrenaline transporter
• NCBI Official Synonym Full Names: solute carrier family 6 member 2
• NCBI Official Symbol: SLC6A2
• NCBI Official Synonym Symbols: NET; NAT1; NET1; SLC6A5
• NCBI Protein Information: sodium-dependent noradrenaline transporter
• UniProt Protein Name: Sodium-dependent noradrenaline transporter
• UniProt Gene Name: SLC6A2
• UniProt Synonym Gene Names: NAT1; NET1; SLC6A5; NET

NCBI Description

This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

Uniprot Description

Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals.