General Alanine Aminotransferase (ALT) Activity (Colorimetric) Assay Kit
Alanine Aminotransferase (ALT) Activity Assay Kit (Colorimetric)
Reactivity
General
Synonyms
Alanine Aminotransferase (ALT) Activity (Colorimetric); Alanine Aminotransferase (ALT) Activity Assay Kit (Colorimetric); Alanine Aminotransferase (ALT) Activity (Colorimetric) assay kit
Reactivity
General
Preparation and Storage
Upon receipt, store the 10X Assay Buffer at 4 degree C. Store the remaining components at -20 degree C.
Related Product Information for Alanine Aminotransferase (ALT) Activity (Colorimetric) assay kit
Principle of the Assay: The Alanine Aminotransferase (ALT) Assay Kit measures ALT activity through a series of enzyme driven reactions. ALT in samples reacts with alanine to transfer an amino group to another substrate, producing glutamate and pyruvate. Pyruvate is then detected with the colorimetric probe. Samples and standards are incubated for 30-60 minutes and then read with a standard 96-well specrophotometric plate reader (540-570 nm). The ALT activity is directly proportional to the amount of pyruvate generated in the reaction. Sample ALT levels are determined by comparison with the known pyruvate standards.
Background/Introduction: Alanine Aminotransferase (ALT), also known as serum glutamic-pyruvic transaminase (SGPT), is an enzyme that catalyzes the transfer of an amino group from alanine to alpha-ketoglutarate, thereby producing glutamate and pyruvate (Figure 1). This pyridoxal phosphate dependent transaminase is found primarily in serum and the liver, but it can also be found in various other body tissues. ALT is usually measured as a clinical marker of liver function to ascertain liver health. Hepatocellular injury often leads to an increase in ALT levels, which are usually measured in units per liter (U/L). The Alanine Aminotransferase (ALT) Assay Kit is a simple colorimetric assay that measures the activity of ALT present within plasma, serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and samples. An ALT positive control is also provided.
Background/Introduction: Alanine Aminotransferase (ALT), also known as serum glutamic-pyruvic transaminase (SGPT), is an enzyme that catalyzes the transfer of an amino group from alanine to alpha-ketoglutarate, thereby producing glutamate and pyruvate (Figure 1). This pyridoxal phosphate dependent transaminase is found primarily in serum and the liver, but it can also be found in various other body tissues. ALT is usually measured as a clinical marker of liver function to ascertain liver health. Hepatocellular injury often leads to an increase in ALT levels, which are usually measured in units per liter (U/L). The Alanine Aminotransferase (ALT) Assay Kit is a simple colorimetric assay that measures the activity of ALT present within plasma, serum, tissue homogenates, or cell suspensions in a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and samples. An ALT positive control is also provided.
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Product Notes
The General Alanine Aminotransferase (ALT) Activity (Colorimetric) (Catalog #AAA169579) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA169579 Assay Kit recognizes General Alanine Aminotransferase (ALT) Activity (Colorimetric). It is sometimes possible for the material contained within the vial of "Alanine Aminotransferase (ALT) Activity (Colorimetric), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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