SDS-Page
Growth Differentiation Factor 11 (GDF11) Recombinant Protein | GDF11 recombinant protein
Recombinant Growth Differentiation Factor 11 (GDF11)
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Growth Differentiation Factor 11 (GDF11); Recombinant Growth Differentiation Factor 11 (GDF11); GDF11 recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CCHFHF SPKVMFTKVL KAQLWVYLRP VPRPATVYLQ ILRLKPLTGE GTAGGGGGGR RHIRIRSLKI ELHSRSGHWQ SIDFKQVLHS WFRQPQSNWG IEINAFDPSG TDLAVTSLGP GAEGCHPFME LRVLENTKRS RRNLGLDCDE HSSESRCCRY PLTVDFEASG WDWIIAPKRY KANYCSGQCEYMFMQKYPHT HLVQQANPRG SAGPCCTPTK MSPINMLYFN DKQQIIYGKI P
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CCHFHF SPKVMFTKVL KAQLWVYLRP VPRPATVYLQ ILRLKPLTGE GTAGGGGGGR RHIRIRSLKI ELHSRSGHWQ SIDFKQVLHS WFRQPQSNWG IEINAFDPSG TDLAVTSLGP GAEGCHPFME LRVLENTKRS RRNLGLDCDE HSSESRCCRY PLTVDFEASG WDWIIAPKRY KANYCSGQCEYMFMQKYPHT HLVQQANPRG SAGPCCTPTK MSPINMLYFN DKQQIIYGKI P
Sequence Length
345
Applicable Applications for GDF11 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Rattus norvegicus (Rat)
Expression System
Prokaryotic expression
Residues
Cys105~Pro341 (Accession # Q9Z217) with two N-terminal Tags, His-tag and T7-tag
Subcellular Location
Secreted
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
9.4
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
30.8kDa
NCBI Official Full Name
Growth/differentiation factor 11
UniProt Protein Name
Growth/differentiation factor 11
Protein Family
UniProt Gene Name
Gdf11
UniProt Synonym Gene Names
Bmp11; GDF-11; BMP-11
UniProt Entry Name
GDF11_RAT
Uniprot Description
Function: Secreted signal that acts globally to specify positional identity along the anterior/posterior axis during development. Play critical roles in patterning both mesodermal and neural tissues and in establishing the skeletal pattern
By similarity.
Subunit structure: Homodimer; disulfide-linked
By similarity.
Subcellular location: Secreted
Probable.
Sequence similarities: Belongs to the TGF-beta family.
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Product Notes
The GDF11 gdf11 (Catalog #AAA2018447) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Growth Differentiation Factor 11 (GDF11) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the GDF11 gdf11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CCHFHF SPKVMFTKVL KAQLWVYLRP VPRPATVYLQ ILRLKPLTGE GTAGGGGGGR RHIRIRSLKI ELHSRSGHWQ SIDFKQVLHS WFRQPQSNWG IEINAFDPSG TDLAVTSLGP GAEGCHPFME LRVLENTKRS RRNLGLDCDE HSSESRCCRY PLTVDFEASG WDWIIAPKRY KANYCSGQCE YMFMQKYPHT HLVQQANPRG SAGPCCTPTK MSPINMLYFN DKQQIIYGKI P. It is sometimes possible for the material contained within the vial of "Growth Differentiation Factor 11 (GDF11), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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