Rabbit anti-Human GAS2L1 Polyclonal Antibody | anti-GAS2L1 antibody

GAS2L1, CT (GAS2L1, GAR22, GAS2-like protein 1, GAS2-related protein on chromosome 22, Growth arrest-specific protein 2-like 1) (Azide free) (HRP)

Cat. Num. AAA6301284

• Gene Names: GAS2L1; GAR22
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GAS2L1; Polyclonal Antibody; CT (GAS2L1; GAR22; GAS2-like protein 1; GAS2-related protein on chromosome 22; Growth arrest-specific protein 2-like 1) (Azide free) (HRP); Growth arrest-specific protein 2-like 1; anti-GAS2L1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GAS2L1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GAS2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 625-654 amino acids from the C-terminal region of human GAS2L1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GAS2L1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GAS2L1 antibody detected the GAS2L1 protein (arrow).)

Flow Cytometry (FC/FACS)

(GAS2L1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GAS2L1 antibody

The protein encoded by this gene, a member of the GAS2 family, is similar in sequence to the mouse protein Gas2, an actin-associated protein expressed at high levels in growth-arrested cells. Expression of the mouse Gas2 gene is negatively regulated by serum and growth factors. Three transcript variants encoding two different isoforms have been found for this gene.

Product Categories/Family for anti-GAS2L1 antibody

Antibodies; Proteins

References

Saito, A., et al. Psychiatry Res (2010). In press: Gamper, I., et al. Exp. Hematol. 37(5):539-548(2009). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Goriounov, D., et al. J. Cell. Sci. 116 (PT 6), 1045-1058 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 23065526
• NCBI GeneID: 10634
• NCBI Accession #: NP_689422.1; NP_689423.1; NP_006469.2
• NCBI GenBank Nucleotide #: NM_152236.2
• UniProt Accession #: Q99501; Q53EN7; Q92640; Q9BUY9
• Molecular Weight: 72,717 Da
• NCBI Official Full Name: GAS2-like protein 1 isoform a
• NCBI Official Synonym Full Names: growth arrest-specific 2 like 1
• NCBI Official Symbol: GAS2L1
• NCBI Official Synonym Symbols: GAR22
• NCBI Protein Information: GAS2-like protein 1; GAS2-related protein on chromosome 22; growth arrest-specific protein 2-like 1
• UniProt Protein Name: GAS2-like protein 1
• Protein Family: GAS2-like protein
• UniProt Gene Name: GAS2L1
• UniProt Synonym Gene Names: GAR22
• UniProt Entry Name: GA2L1_HUMAN

NCBI Description

This gene encodes a member of the growth arrest-specific 2 protein family. This protein binds components of the cytoskeleton and may be involved in mediating interactions between microtubules and microfilaments. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 9. [provided by RefSeq, Jul 2013]

Uniprot Description

GAS2L1: Seems to be involved in the cross-linking of microtubules and microfilaments. Belongs to the GAS2 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: microtubule; cytoskeleton; cytoplasm; stress fiber

Molecular Function: microtubule binding; cytoskeletal adaptor activity; thyroid hormone receptor binding

Biological Process: negative regulation of erythrocyte differentiation; regulation of cell cycle; negative regulation of microtubule depolymerization; negative regulation of cell growth; cell cycle arrest; cellular response to starvation; microtubule bundle formation