Porcine Growth arrest-specific gene 6 ELISA Kit | GAS6 elisa kit

Porcine Growth arrest-specific gene 6 ELISA Kit

Cat. Num. AAA751094

• Gene Names: GAS6; AXSF; AXLLG
• Reactivity: Porcine
• Synonyms: Growth arrest-specific gene 6; Porcine Growth arrest-specific gene 6 ELISA Kit; GAS6 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This GAS6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GAS6. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This GAS6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GAS6. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GAS6 elisa kit

Principle of the Assay: GAS6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GAS6 antibody and an GAS6-HRP conjugate. The assay sample and buffer are incubated together with GAS6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAS6 concentration since GAS6 from samples and GAS6-HRP conjugate compete for the anti-GAS6 antibody binding site. Since the number of sites is limited, as more sites are occupied by GAS6 from the sample, fewer sites are left to bind GAS6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAS6 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557617
• NCBI GeneID: 2621
• NCBI Accession #: NP_000811.1
• NCBI GenBank Nucleotide #: NM_000820.3
• Molecular Weight: 41,986 Da
• NCBI Official Full Name: growth arrest-specific protein 6
• NCBI Official Synonym Full Names: growth arrest-specific 6
• NCBI Official Symbol: GAS6
• NCBI Official Synonym Symbols: AXSF; AXLLG
• NCBI Protein Information: growth arrest-specific protein 6; AXL stimulatory factor; AXL receptor tyrosine kinase ligand
• UniProt Protein Name: Growth arrest-specific protein 6
• Protein Family: Growth arrest-specific protein
• UniProt Gene Name: GAS6
• UniProt Synonym Gene Names: AXLLG; GAS-6
• UniProt Entry Name: GAS6_HUMAN

NCBI Description

This gene encodes a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. [provided by RefSeq, Aug 2014]

Uniprot Description

GAS6: Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; cytoplasm; extracellular region

Molecular Function: voltage-gated calcium channel activity; protein binding; phosphatidylserine binding; caspase inhibitor activity; protein tyrosine kinase activator activity; calcium ion binding; receptor tyrosine kinase binding; receptor agonist activity; molecular adaptor activity; receptor binding

Biological Process: proteolysis; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; positive regulation of fibroblast proliferation; viral envelope fusion with host membrane; negative regulation of protein import into nucleus, translocation; regulation of growth; positive regulation of phagocytosis; negative regulation of interleukin-6 production; cell cycle arrest; cell adhesion; positive regulation of TOR signaling pathway; platelet activation; organ regeneration; negative regulation of transcription factor activity; activation of protein kinase B; cellular response to starvation; peptidyl-glutamic acid carboxylation; negative regulation of transcription, DNA-dependent; leukocyte migration; negative regulation of interleukin-1 secretion; negative regulation of apoptosis; entry of virus into host cell; neuron migration; negative regulation of caspase activity; signal transduction; positive regulation of protein export from nucleus; positive regulation of natural killer cell differentiation; platelet degranulation; positive regulation of glomerular filtration; negative regulation of interferon-gamma production; protein kinase B signaling cascade; virion attachment, binding of host cell surface receptor; cell migration; negative regulation of tumor necrosis factor production; viral genome replication; post-translational protein modification; positive regulation of peptidyl-serine phosphorylation; cell-substrate adhesion; phagocytosis; cell proliferation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; cellular protein metabolic process; positive regulation of protein kinase activity; positive regulation of protein amino acid phosphorylation; positive regulation of cytokine and chemokine mediated signaling pathway; blood coagulation; apoptotic cell clearance