Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse brain tissue, Mouse lung tissue, Primary antibody: 162300(SPON1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)
Rabbit anti-Human, Mouse SPON1 Polyclonal Antibody | anti-SPON1 antibody
SPON1 Rabbit pAb
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
SPON1; Polyclonal Antibody; SPON1 Rabbit pAb; spondin 1; extracellular matrix protein; f-spondin; VSGP/F-spondin; anti-SPON1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-SPON1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:200-1:1000
IHC: 1:25-1:100
IHC: 1:25-1:100
Immunogen
Synthetic peptide of human SPON1
Conjugation
Unconjugated
Modification
Unmodified
Cell Position
Extracellular matrix, Secreted
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse brain tissue, Mouse lung tissue, Primary antibody: 162300(SPON1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse brain tissue, Mouse lung tissue, Primary antibody: 162300(SPON1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 162300(SPON1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))
Immunohistochemistry (IHC)
(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 162300(SPON1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200))
Product Categories/Family for anti-SPON1 antibody
NCBI and Uniprot Product Information
Uniprot Description
Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (). Major factor for vascular smooth muscle cell.
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Product Notes
The SPON1 spon1 (Catalog #AAA4754903) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPON1 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPON1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:200-1:1000 IHC: 1:25-1:100. Researchers should empirically determine the suitability of the SPON1 spon1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPON1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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