Growth Arrest and DNA-Damage-Inducible Alpha Recombinant Protein | GADD45A recombinant protein

Recombinant Human Growth Arrest and DNA-Damage-Inducible Alpha

Cat. Num. AAA143853

• Gene Names: GADD45A; DDIT1; GADD45
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Synonyms: Growth Arrest and DNA-Damage-Inducible Alpha; Recombinant Human Growth Arrest and DNA-Damage-Inducible Alpha; GADD45A Human; Growth Arrest and DNA-Damage-Inducible Alpha Human Recombinant; Growth arrest and DNA damage-inducible protein GADD45 alpha; DNA damage-inducible transcript 1 protein; DDIT-1; GADD45A; DDIT1; GADD45; GADD45A recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by SDS-PAGE.
• Form/Format: The GADD45A protein solution contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.1M NaCl.; Sterile filtered colorless solution.
• Sequence: MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA ADEDDDRDVA LQIHFTLIQA FCCENDINILRVSNPGRLAE LLLLETDAGP AASEGAEQPP DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPERLEHHH HHH
• Sequence Length: 131

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for GADD45A recombinant protein

Description: GADD45A produced in E Coli is a single, non-glycosylated polypeptide chain containing 173 amino acids (1-165 a.a.) and having a molecular mass of 19.4kDa. GADD45A is fused to 8 amino acids His Tag at C-terminus and purified by proprietary chromatographic techniques.

Introduction: Growth arrest and DNA Damage-Inducible Protein (GADD45A) binds both Cdks and PCNA. GADD45A is involved in DNA replication and repair. GADD45A stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A may serve as a link between p53-dependent cell cycle checkpoint and DNA repair. The GADD45A gene belongs to a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. GADD45A binds to proliferating cell nuclear antigen.

Product Categories/Family for GADD45A recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; DNA-Damage Protein

NCBI and Uniprot Product Information

• NCBI GI #: 315075323
• NCBI GeneID: 1647
• NCBI Accession #: NP_001186670.1
• NCBI GenBank Nucleotide #: NM_001199741.1
• UniProt Accession #: P24522; Q5TCA7; Q5TCA8
• Molecular Weight: 14,622 Da
• NCBI Official Full Name: growth arrest and DNA damage-inducible protein GADD45 alpha isoform 2
• NCBI Official Synonym Full Names: growth arrest and DNA-damage-inducible, alpha
• NCBI Official Symbol: GADD45A
• NCBI Official Synonym Symbols: DDIT1; GADD45
• NCBI Protein Information: growth arrest and DNA damage-inducible protein GADD45 alpha; DDIT-1; DNA damage-inducible transcript 1 protein; DNA damage-inducible transcript-1; DNA-damage-inducible transcript 1; growth arrest and DNA-damage-inducible 45 alpha
• UniProt Protein Name: Growth arrest and DNA damage-inducible protein GADD45 alpha
• Protein Family: Growth arrest and DNA damage-inducible protein
• UniProt Gene Name: GADD45A
• UniProt Synonym Gene Names: DDIT1; GADD45; DDIT-1
• UniProt Entry Name: GA45A_HUMAN

NCBI Description

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]

Uniprot Description

GADD45A: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Interacts with MAPK14. Interacts with GADD45GIP1. Interacts with PCNA. By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC. Belongs to the GADD45 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Apoptosis; DNA repair, damage; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p31.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: apoptosis; positive regulation of apoptosis; centrosome cycle; activation of MAPKKK activity; DNA damage response, signal transduction; negative regulation of protein kinase activity; positive regulation of JNK cascade; regulation of cyclin-dependent protein kinase activity; DNA repair; cell cycle arrest