GADD45A recombinant protein

GADD45A, 1-165aa, Human, His tag, E Coli

Cat. Num. AAA203413

• Gene Names: GADD45A; DDIT1; GADD45
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: GADD45A; 1-165aa; Human; His tag; E Coli; Growth arrest and DNA damage-inducible protein GADD45 alpha; DDIT1; GADD45; Growth arrest and DNA damage-inducible protein GADD45 alpha DDIT 1; DNA damage inducible transcript 1; Growth arrest and DNA damage inducible 45 alpha; ; GADD45A recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA ADEDDDRDVA LQIHFTLIQA FCCENDINIL RVSNPGRLAE LLLLETDAGP AASEGAEQPP DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPERLEHHH HHH
• Sequence Length: 165

• Applicable Applications for GADD45A recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GADD45A recombinant protein

GADD45A, also known as growth arrest and DNA damage-inducible protein, binds both Cdks and PCNA, a protein involved in DNA replication and repair. It has been shown to stimulate DNA excision repair in vitro and to inhibit entry of cells into S phase. This protein may serve as a link between p53-dependent cell cycle checkpoint and DNA repair. Recombinant human GADD45A protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for GADD45A recombinant protein

Cell Cycle

References

Hollander MC. et al. (1993) J Biol Chem. 268 (32):24385-93; Takekawa M. et al. (1998) Cell. 95 (4):521-30.;

NCBI and Uniprot Product Information

• NCBI GI #: 4503287
• NCBI GeneID: 1647
• NCBI Accession #: NP_001915
• NCBI GenBank Nucleotide #: NM_001924.3
• UniProt Accession #: P24522; Q5TCA7; Q5TCA8
• Molecular Weight: 19.4 kDa (173aa), confirmed by MALDI-TOF
• NCBI Official Full Name: growth arrest and DNA damage-inducible protein GADD45 alpha isoform 1
• NCBI Official Synonym Full Names: growth arrest and DNA damage inducible alpha
• NCBI Official Symbol: GADD45A
• NCBI Official Synonym Symbols: DDIT1; GADD45
• NCBI Protein Information: growth arrest and DNA damage-inducible protein GADD45 alpha
• UniProt Protein Name: Growth arrest and DNA damage-inducible protein GADD45 alpha
• Protein Family: Growth arrest and DNA damage-inducible protein
• UniProt Gene Name: GADD45A
• UniProt Synonym Gene Names: DDIT1; GADD45; DDIT-1

NCBI Description

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]

Uniprot Description

In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.