PABPN1 recombinant protein

PABPN1, 119-306aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205594

• Gene Names: PABPN1; OPMD; PAB2; PABII; PABP2; PABP-2
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PABPN1; 119-306aa; Human; His tag; E Coli (Denatured); Polyadenylate-binding protein 2; OPMD; PAB2; PABII; PABP-2; PABP2; PABPN1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSLEAIKAR VREMEEEAEK LKELQNEVEK QMNMSPPPGN AGPVIMSIEE KMEADARSIY VGNVDYGATA EELEAHFHGC GSVNRVTILC DKFSGHPKGF AYIEFSDKES VRTSLALDES LFRGRQIKVI PKRTNRPGIS TTDRGFPRAR YRARTTNYNS SRSRFYSGFN SRPRGRVYRG RARATSWYSP Y
• Sequence Length: 306

• Applicable Applications for PABPN1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PABPN1 recombinant protein

PABPN1 is an abundant nuclear protein that binds with high affinity to nascent poly (A) tails. The protein is required for progressive and efficient polymerization of poly (A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly (A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly (A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene. Recombinant human PABPN1 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for PABPN1 recombinant protein

Epigenomics (Transcription & Translation)

References

Fan X., et al. (2001) Hum. Mol. Genet. 10:2341-2351.

NCBI and Uniprot Product Information

• NCBI GI #: 4758876
• NCBI GeneID: 8106
• NCBI Accession #: NP_004634
• NCBI GenBank Nucleotide #: NM_004643.3
• UniProt Accession #: Q86U42; O43484; D3DS49
• Molecular Weight: 23.8 kDa (211aa)
• NCBI Official Full Name: polyadenylate-binding protein 2 isoform 1
• NCBI Official Synonym Full Names: poly(A) binding protein nuclear 1
• NCBI Official Symbol: PABPN1
• NCBI Official Synonym Symbols: OPMD; PAB2; PABII; PABP2; PABP-2
• NCBI Protein Information: polyadenylate-binding protein 2
• UniProt Protein Name: Polyadenylate-binding protein 2
• UniProt Gene Name: PABPN1
• UniProt Synonym Gene Names: PAB2; PABP2; PABP-2; Poly(A)-binding protein 2; PABII

NCBI Description

This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. This gene contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between this gene and the neighboring upstream BCL2-like 2 (BCL2L2) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product (). Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length (). Increases the affinity of poly(A) polymerase for RNA (). Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506). Binds to poly(A) and to poly(G) with high affinity (). May protect the poly(A) tail from degradation (). Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484).