Mouse coagulation Factor XII ELISA Kit | FXII elisa kit

Mouse coagulation Factor XII ELISA Kit

Cat. Num. AAA725976

• Gene Names: F12; FXII
• Reactivity: Mouse
• Synonyms: coagulation Factor XII; Mouse coagulation Factor XII ELISA Kit; Mouse coagulation Factor XII (FXII) ELISA Kit; coagulation Factor XII (Mouse); FXII elisa kit

• Reactivity: Mouse

• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FXII elisa kit

Intended Uses: This FXII ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of FXII in the sample, this FXII ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus FXII concentration. The concentration of FXII in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This FXII enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for FXII. Standards or samples are then added to the microtiter plate wells and FXII if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FXII present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FXII are added to each well to "sandwich" the FXII immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain FXII and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for FXII elisa kit

Mouse ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 4165317
• NCBI GeneID: 397474
• UniProt Accession #: O97507
• Molecular Weight: 68,012 Da
• NCBI Official Full Name: FXII
• NCBI Official Symbol: F12
• NCBI Official Synonym Symbols: FXII
• NCBI Protein Information: coagulation factor XII; HAF; hageman factor
• UniProt Protein Name: Coagulation factor XII
• Protein Family: Coagulation factor
• UniProt Gene Name: F12
• UniProt Entry Name: FA12_PIG

Uniprot Description

Function: Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa

Catalytic activity: Selective cleavage of Arg-|-Ile bonds in factor VII to form factor VIIa and factor XI to form factor XIa.

Subunit structure: Interacts with HRG; the interaction, which is enhanced in the presence of zinc ions and inhibited by heparin-binding, inhibits factor XII autoactivation and contact-initiated coagulation

Subcellular location: Secreted.

Post-translational modification: O- and N-glycosylated

Sequence similarities: Belongs to the peptidase S1 family.Contains 2 EGF-like domains.Contains 1 fibronectin type-I domain.Contains 1 fibronectin type-II domain.Contains 1 kringle domain.Contains 1 peptidase S1 domain.