Typical Standard Curve/Testing Data
Human coagulation Factor XII ELISA Kit | FXII elisa kit
Human coagulation Factor XII ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: FXII ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FXII antibody and an FXII-HRP conjugate. The assay sample and buffer are incubated together with FXII-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FXII concentration since FXII from samples and FXII-HRP conjugate compete for the anti-FXII antibody binding site. Since the number of sites is limited, as more sites are occupied by FXII from the sample, fewer sites are left to bind FXII-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FXII concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
Function: Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa
Catalytic activity: Selective cleavage of Arg-|-Ile bonds in factor VII to form factor VIIa and factor XI to form factor XIa.
Subunit structure: Interacts with HRG; the interaction, which is enhanced in the presence of zinc ions and inhibited by heparin-binding, inhibits factor XII autoactivation and contact-initiated coagulation
Subcellular location: Secreted.
Post-translational modification: O- and N-glycosylated
Sequence similarities: Belongs to the peptidase S1 family.Contains 2 EGF-like domains.Contains 1 fibronectin type-I domain.Contains 1 fibronectin type-II domain.Contains 1 kringle domain.Contains 1 peptidase S1 domain.
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Product Notes
The Human FXII f12 (Catalog #AAA729006) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA729006 ELISA Kit recognizes Human FXII. It is sometimes possible for the material contained within the vial of "coagulation Factor XII, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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