IDH3G recombinant protein

IDH3G, 40-393aa, Human, His tag, E Coli

Cat. Num. AAA204556

• Gene Names: IDH3G; H-IDHG
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: IDH3G; 40-393aa; Human; His tag; E Coli; Isocitrate dehydrogenase [NAD] subunit gamma mitochondrial; Isocitrate dehydrogenase [NAD] subunit gamma; mitochondrial; H-IDHG; IDH3G recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl, 5mM DTT, 2mM EDTA
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MFSEQTIPPS AKYGGRHTVT MIPGDGIGPE LMLHVKSVFR HACVPVDFEE VHVSSNADEE DIRNAIMAIR RNRVALKGNI ETNHNLPPSH KSRNNILRTS LDLYANVIHC KSLPGVVTRH KDIDILIVRE NTEGEYSSLE HESVAGVVES LKIITKAKSL RIAEYAFKLA QESGRKKVTA VHKANIMKLG DGLFLQCCRE VAARYPQITF ENMIVDNTTM QLVSRPQQFD VMVMPNLYGN IVNNVCAGLV GGPGLVAGAN YGHVYAVFET ATRNTGKSIA NKNIANPTAT LLASCMMLDH LKLHSYATSI RKAVLASMDN ENMHTPDIGG QGTTSEAIQD VIRHIRVING RAVEA
• Sequence Length: 393

• Applicable Applications for IDH3G recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for IDH3G recombinant protein

IDH3G, also known as isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, belongs to the isocitrate and isopropylmalate dehydrogenases family. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH3G is a gamma subunit of one isozyme of isocitrate dehydrogenase that belongs to a distinct subclass, which utilizes NAD (+) as the electron acceptor, and is localised to the mitochondrial matrix. Recombinant human IDH3G protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for IDH3G recombinant protein

Metabolism

References

Kim Y.-O., et al. (1999) J. Biol. Chem. 274:36866-36875; Brenner V., et al. (1997) Genomics. 44:8-14

NCBI and Uniprot Product Information

• NCBI GI #: 4758582
• NCBI GeneID: 3421
• NCBI Accession #: NP_004126
• NCBI GenBank Nucleotide #: NM_004135.3
• UniProt Accession #: P51553; Q9BUU5; E9PDD5
• Molecular Weight: 41.1 kDa (375aa), confirmed by MALDI-TOF
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 3 (NAD(+)) gamma
• NCBI Official Symbol: IDH3G
• NCBI Official Synonym Symbols: H-IDHG
• NCBI Protein Information: isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
• UniProt Protein Name: Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH3G

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Regulatory subunit which plays a role in the allosteric regulation of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.