FSTL5 recombinant protein

FSTL5 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8121399

• Purity: >85% as determined by SDS-PAGE
• Synonyms: FSTL5; FSTL5 Protein; Human; Recombinant (hFc Tag); Human FSTL5 Protein (Fc Tag); KIAA1263 Protein; follistatin-like 5; FSTL5 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala847

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Arg 21
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human FSTL5 (Q8N475-1)(Met1-Ala847) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FSTL5 recombinant protein

Background: FSTL5 may have molecular function (calcium ion binding) and to localize in various compartments (cytoplasm, extracellular space, extracellular region). FSTL5 expression denoted a dismal prognosis both within and across medulloblastoma subgroups. FSTL5 gene is well expressed, 1.0 times the average gene in this release. The sequence of this gene is defined by 120 GenBank accessions from 113 cDNA clones, some from brain, cerebellum, eye, melanotic melanoma, skin, amygdala, breast and 24 other tissues. FSTL5 gene contains 27 distinct introns. The addition of FSTL5 immunohistochemistry to existing molecular stratification schemes constitutes a reliable and cost-effective tool for prognostication in future clinical trials of medulloblastoma.

References

Masuda T, et al. (2009) Laser capture microdissection and cDNA array analysis for identification of mouse KIAA/FLJ genes differentially expressed in the embryonic dorsal spinal cord. Brain Res. 1249:61-7. Kingwell K. (2011) FSTL5--a new prognostic biomarker for medulloblastoma. Nat Rev Neurol. 7(11):598. Remke M, et al. (2011) FSTL5 is a marker of poor prognosis in non-WNT/non-SHH medulloblastoma. J Clin Oncol. 29(29):3852-61.

NCBI and Uniprot Product Information

• NCBI GI #: 190358524
• NCBI GeneID: 56884
• NCBI Accession #: NP_001121900.1
• NCBI GenBank Nucleotide #: NM_001128428.2
• UniProt Accession #: Q8N475; Q9NSW7; Q9ULF7; E9PCP6
• Molecular Weight: 95,623 Da
• NCBI Official Full Name: follistatin-related protein 5 isoform c
• NCBI Official Synonym Full Names: follistatin like 5
• NCBI Official Symbol: FSTL5
• NCBI Protein Information: follistatin-related protein 5
• UniProt Protein Name: Follistatin-related protein 5
• Protein Family: Follistatin-related protein
• UniProt Gene Name: FSTL5
• UniProt Synonym Gene Names: KIAA1263