Mouse follistatin ELISA Kit | FST elisa kit

Mouse Follistatin, FS ELISA Kit

Cat. Num. AAA703755

• Gene Names: Fst; FS; AL033346
• Reactivity: Mouse
• Synonyms: follistatin; Mouse Follistatin; FS ELISA Kit; FS; follistatin isoform FST317; FST elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse FST. No significant cross-reactivity or interference between Mouse FST and analogues was observed.
• Sequence Length: 343

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Competitive
• Detection Range: 0.05 ng/ml-10 ng/ml
• Sensitivity: 0.025ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FST elisa kit

For samples:For the quantitative determination of mouse follistatin (FS) concentrations in serum, plasma, tissue homogenates, cell lysates.

PRINCIPLE OF THE ASSAY This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for follistatin and Horseradish Peroxidase (HRP) conjugated follistatin. The competitive inhibition reaction is launched between with HRP labeled follistatin and unlabeled follistatin with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of follistatin in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6679867
• NCBI GeneID: 14313
• NCBI Accession #: NP_032072.1
• NCBI GenBank Nucleotide #: NM_008046.3
• UniProt Accession #: P47931; A6H6P0
• Molecular Weight: 37,866 Da
• NCBI Official Full Name: follistatin isoform 2
• NCBI Official Synonym Full Names: follistatin
• NCBI Official Symbol: Fst
• NCBI Official Synonym Symbols: FS; AL033346
• NCBI Protein Information: follistatin; activin-binding protein
• UniProt Protein Name: Follistatin
• Protein Family: Follistatin
• UniProt Gene Name: Fst
• UniProt Synonym Gene Names: FS
• UniProt Entry Name: FST_MOUSE

NCBI Description

The protein encoded by this gene binds to and negatively regulates activin, as well as other members of the transforming growth factor beta family, and acts to prevent uncontrolled cellular proliferation. This protein also contains a heparin-binding sequence. It is expressed in many of the tissues in which activin is synthesized and is thought to clear activin from the circulation by attachment to the cell surface. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms, including FST315 and FST288, that differ at their C-terminus. Another isoform, FST303 is thought to be produced by proteolytic cleavage of FST315. These isoforms differ in their localization and in their ability to bind heparin. While FST315 is a circulating protein, FST288 is tissue-bound, and FST303 is gonad-specific. While deletion of all isoforms results in embryonic lethality, expression of just FST288 is sufficient for embryonic development, but the resultant mice have fertility defects. [provided by RefSeq, Aug 2014]

Uniprot Description

Function: Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).