Sheep Follistatin ELISA Kit | FS elisa kit

Sheep Follistatin ELISA Kit

Cat. Num. AAA740217

• Gene Names: Fst; FS; AL033346
• Reactivity: Sheep
• Synonyms: Follistatin; Sheep Follistatin ELISA Kit; FS elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This FS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep FS. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FS elisa kit

Principle of the assay: FS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FS antibody and an FS-HRP conjugate. The assay sample and buffer are incubated together with FS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FS concentration since FS from samples and FS-HRP conjugate compete for the anti-FS antibody binding site. Since the number of sites is limited, as more sites are occupied by FS from the sample, fewer sites are left to bind FS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FS concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FS elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 6679867
• NCBI GeneID: 14313
• NCBI Accession #: NP_032072.1
• NCBI GenBank Nucleotide #: NM_008046.3
• Molecular Weight: 37,866 Da
• NCBI Official Full Name: follistatin isoform 2
• NCBI Official Synonym Full Names: follistatin
• NCBI Official Symbol: Fst
• NCBI Official Synonym Symbols: FS; AL033346
• NCBI Protein Information: follistatin; activin-binding protein
• UniProt Protein Name: Follistatin
• Protein Family: Follistatin
• UniProt Gene Name: Fst
• UniProt Synonym Gene Names: FS
• UniProt Entry Name: FST_MOUSE

NCBI Description

The protein encoded by this gene binds to and negatively regulates activin, as well as other members of the transforming growth factor beta family, and acts to prevent uncontrolled cellular proliferation. This protein also contains a heparin-binding sequence. It is expressed in many of the tissues in which activin is synthesized and is thought to clear activin from the circulation by attachment to the cell surface. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms, including FST315 and FST288, that differ at their C-terminus. Another isoform, FST303 is thought to be produced by proteolytic cleavage of FST315. These isoforms differ in their localization and in their ability to bind heparin. While FST315 is a circulating protein, FST288 is tissue-bound, and FST303 is gonad-specific. While deletion of all isoforms results in embryonic lethality, expression of just FST288 is sufficient for embryonic development, but the resultant mice have fertility defects. [provided by RefSeq, Aug 2014]

Uniprot Description

FST: Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH)

Protein type: Secreted, signal peptide; Secreted

Cellular Component: cytoplasm; extracellular region; nucleus

Molecular Function: heparan sulfate proteoglycan binding; activin binding

Biological Process: BMP signaling pathway; positive regulation of hair follicle development; negative regulation of activin receptor signaling pathway; negative regulation of cell differentiation; hair follicle morphogenesis; gamete generation; hemopoietic progenitor cell differentiation; keratinocyte proliferation; negative regulation of transcription from RNA polymerase II promoter; skeletal development; pattern specification process; female gonad development; odontogenesis of dentine-containing teeth