Human Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) ELISA Kit | SCNN1b elisa kit

Human Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) ELISA Kit

Cat. Num. AAA451598

• Gene Names: SCNN1B; BESC1; ENaCb; SCNEB; ENaCbeta
• Reactivity: Human
• Synonyms: Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b); Human Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) ELISA Kit; ENaCb; ENaCbeta; SCNEB; Liddle Syndrome; Sodium Channel; Nonvoltage-Gated 1; Beta; Epithelial Na(+) channel subunit beta; Nonvoltage-gated sodium channel 1 subunit beta; SCNN1b elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SCNN1b. No significant cross-reactivity or interference between SCNN1b and analogues was observed.
• Sequence Length: 640

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.107ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SCNN1b were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SCNN1b were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCNN1b elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SCNN1b in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SCNN1b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SCNN1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SCNN1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SCNN1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 124301196
• NCBI GeneID: 6338
• NCBI Accession #: NP_000327.2
• NCBI GenBank Nucleotide #: NM_000336.2
• UniProt Accession #: P51168; O60891; Q96KG2; Q9UJ32; Q9UMU5; C5HTZ2
• Molecular Weight: 77,703 Da
• NCBI Official Full Name: amiloride-sensitive sodium channel subunit beta
• NCBI Official Synonym Full Names: sodium channel epithelial 1 beta subunit
• NCBI Official Symbol: SCNN1B
• NCBI Official Synonym Symbols: BESC1; ENaCb; SCNEB; ENaCbeta
• NCBI Protein Information: amiloride-sensitive sodium channel subunit beta
• UniProt Protein Name: Amiloride-sensitive sodium channel subunit beta
• Protein Family: Amiloride-sensitive sodium channel
• UniProt Gene Name: SCNN1B
• UniProt Synonym Gene Names: Beta-ENaC; ENaCB

NCBI Description

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the beta subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), and Liddle syndrome. [provided by RefSeq, Apr 2009]

Uniprot Description

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.