Immunofluorescence (IF)
(Immunofluorescence staining of SOCS1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human SOCS1 polyclonal antibody (1:1000)(Figure A), incubated with rabbit anti-human SOCS1 polyclonal antibody and antigen (Figure B) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)
Rabbit SOCS1 Polyclonal Antibody | anti-SOCS1 antibody
Anti-SOCS1 Antibody, Rabbit Polyclonal
Gene Names
SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
Reactivity
HumanPredicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
SOCS1; Polyclonal Antibody; Anti-SOCS1 Antibody; Rabbit Polyclonal; SOCS1 Antibody; Rabbit PAb; Antigen Affinity Purified; suppressor of cytokine signaling 1; Anti-CIS1 Antibody; Anti-CISH1 Antibody; Anti-JAB Antibody; Anti-SOCS-1 Antibody; Anti-SSI-1 Antibody; Anti-SSI1 Antibody; Anti-TIP3 Antibody; anti-SOCS1 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SOCS1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SOCS1 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:300-1:10,000
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human SOCS1
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human SOCS1, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of SOCS1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human SOCS1 polyclonal antibody (1:1000)(Figure A), incubated with rabbit anti-human SOCS1 polyclonal antibody and antigen (Figure B) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of SOCS1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human SOCS1 polyclonal antibody (1:1000)(Figure A), incubated with rabbit anti-human SOCS1 polyclonal antibody and antigen (Figure B) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)
Related Product Information for anti-SOCS1 antibody
Suppressor of cytokine signaling1 (SOCS1) is a widely recognized tumor suppressor gene. Silencing of SOCS1 expression as a result of promoter methylation is associated with occurrence and development of solid tumors such as liver, cervical and pancreatic cancer. Downregulation of suppressor of cytokine signalling-1 (SOCS1) is one of the vital reasons for JAK1-STAT3 pathway activation in acute myeloid leukaemia (AML). The DCs transduced with SOCS1 gene exhibit as DCregs through negative regulation of JAK2/STAT1 pathway and ameliorated lipopolysaccharide/d-galactosamine induced acute liver failure via inhibition of TLR4 pathway.
Product Categories/Family for anti-SOCS1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
211
NCBI Official Full Name
suppressor of cytokine signaling 1
NCBI Official Synonym Full Names
suppressor of cytokine signaling 1
NCBI Official Symbol
SOCS1
NCBI Official Synonym Symbols
JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
NCBI Protein Information
suppressor of cytokine signaling 1; TIP-3; JAK binding protein; JAK-binding protein; Tec-interacting protein 3; STAT induced SH3 protein 1; STAT-induced STAT inhibitor 1; cytokine-inducible SH2 protein 1
UniProt Protein Name
Suppressor of cytokine signaling 1
Protein Family
UniProt Gene Name
SOCS1
UniProt Synonym Gene Names
SSI1; TIP3; SOCS-1; JAB; SSI-1; TIP-3
UniProt Entry Name
SOCS1_HUMAN
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Product Notes
The SOCS1 socs1 (Catalog #AAA8101059) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SOCS1 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's SOCS1 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:300-1:10,000. Researchers should empirically determine the suitability of the SOCS1 socs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOCS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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