Filaggrin (FLG) Recombinant Protein | FLG recombinant protein

Recombinant Filaggrin (FLG)

Cat. Num. AAA2009057

• Gene Names: FLG; ATOD2
• Reactivity: Homo sapiens (Human)
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Filaggrin (FLG); Recombinant Filaggrin (FLG); FLG recombinant protein

• Host: E Coli
• Reactivity: Homo sapiens (Human)
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.05% sarcosyl and preservative.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- SHRDTA SHVQSSPVQS DSSTAKEHGH FSSLSQDSAY HSGIQSRGSP HSSSSYHYQS EGTERQKGQS GLVWRHGSYG SADYDYGESG FRHSQHGSVSYNSNPVVFKE RSDICKASAF GKDHPRYYAT Y
• Sequence Length: 4061

• Applicable Applications for FLG recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Expression System: Prokaryotic expression
• Residues: Ser3905~Tyr4031 (Accession # P20930) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted isoelectric point: 7.0
• Predicted Molecular Mass: 15.5kDa
• Accurate Molecular Mass: 25kDa as determined by SDS-PAGE reducing conditions.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 60097902
• NCBI GeneID: 2312
• NCBI Accession #: NP_002007.1
• NCBI GenBank Nucleotide #: NM_002016.1
• UniProt Accession #: P20930; Q01720; Q5T583; Q9UC71
• NCBI Official Full Name: filaggrin
• NCBI Official Synonym Full Names: filaggrin
• NCBI Official Symbol: FLG
• NCBI Official Synonym Symbols: ATOD2
• NCBI Protein Information: filaggrin; epidermal filaggrin
• UniProt Protein Name: Filaggrin
• Protein Family: Filaggrin
• UniProt Gene Name: FLG
• UniProt Entry Name: FILA_HUMAN

NCBI Description

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]

Uniprot Description

FLG: Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. Defects in FLG are the cause of ichthyosis vulgaris (VI); also known as ichthyosis simplex. Ichthyosis vulgaris is the most common form of ichthyosis inherited as an autosomal dominant trait. It is characterized by palmar hyperlinearity, keratosis pilaris and a fine scale that is most prominent over the lower abdomen, arms, and legs. Ichthyosis vulgaris is characterized histologically by absent or reduced keratohyalin granules in the epidermis and mild hyperkeratosis. The disease can be associated with frequent asthma, eczema or hay fever. Defects in FLG are a cause of susceptibility to dermatitis atopic type 2 (ATOD2). Atopic dermatitis is a complex, inflammatory disease with multiple alleles at several loci thought to be involved in the pathogenesis. It commonly begins in infancy or early childhood and is characterized by a chronic relapsing form of skin inflammation, a disturbance of epidermal barrier function that culminates in dry skin, and IgE- mediated sensitization to food and environmental allergens. It is manifested by lichenification, excoriation, and crusting, mainly on the flexural surfaces of the elbow and knee. Belongs to the S100-fused protein family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytoplasmic membrane-bound vesicle; intermediate filament; nucleus

Molecular Function: protein binding; calcium ion binding; structural molecule activity

Biological Process: keratinocyte differentiation; multicellular organismal development

Disease: Ichthyosis Vulgaris; Dermatitis, Atopic, 2