Human Filaggrin ELISA Kit | FLG elisa kit

Human Filaggrin ELISA Kit

Cat. Num. AAA760777

• Gene Names: FLG; ATOD2
• Reactivity: Human
• Synonyms: Filaggrin; Human Filaggrin ELISA Kit; FLG/Filaggrin/ATOD2; FLG elisa kit

• Reactivity: Human
• Sequence Length: 4061

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FLG elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human FLG antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human FLG antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FLG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human FLG can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 60097902
• NCBI GeneID: 2312
• NCBI Accession #: NP_002007.1
• NCBI GenBank Nucleotide #: NM_002016.1
• Molecular Weight: 435,170 Da
• NCBI Official Full Name: filaggrin
• NCBI Official Synonym Full Names: filaggrin
• NCBI Official Symbol: FLG
• NCBI Official Synonym Symbols: ATOD2
• NCBI Protein Information: filaggrin
• UniProt Protein Name: Filaggrin
• Protein Family: Filaggrin
• UniProt Gene Name: FLG
• UniProt Entry Name: FILA_HUMAN

NCBI Description

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]

Uniprot Description

FLG: Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. Defects in FLG are the cause of ichthyosis vulgaris (VI); also known as ichthyosis simplex. Ichthyosis vulgaris is the most common form of ichthyosis inherited as an autosomal dominant trait. It is characterized by palmar hyperlinearity, keratosis pilaris and a fine scale that is most prominent over the lower abdomen, arms, and legs. Ichthyosis vulgaris is characterized histologically by absent or reduced keratohyalin granules in the epidermis and mild hyperkeratosis. The disease can be associated with frequent asthma, eczema or hay fever. Defects in FLG are a cause of susceptibility to dermatitis atopic type 2 (ATOD2). Atopic dermatitis is a complex, inflammatory disease with multiple alleles at several loci thought to be involved in the pathogenesis. It commonly begins in infancy or early childhood and is characterized by a chronic relapsing form of skin inflammation, a disturbance of epidermal barrier function that culminates in dry skin, and IgE- mediated sensitization to food and environmental allergens. It is manifested by lichenification, excoriation, and crusting, mainly on the flexural surfaces of the elbow and knee. Belongs to the S100-fused protein family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: intermediate filament; intracellular membrane-bound organelle; nucleus

Molecular Function: calcium ion binding; protein binding; structural molecule activity

Biological Process: keratinocyte differentiation; multicellular organismal development

Disease: Dermatitis, Atopic, 2; Ichthyosis Vulgaris