Apoptosis Signal Regulating Kinase 1 (ASK1) Recombinant Protein | ASK1 recombinant protein

Recombinant Apoptosis Signal Regulating Kinase 1 (ASK1)

Cat. Num. AAA2018491

• Gene Names: Map3k5; ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Apoptosis Signal Regulating Kinase 1 (ASK1); Recombinant Apoptosis Signal Regulating Kinase 1 (ASK1); ASK1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LGKGTYGI VYAGRDLSNQ VRIAIKEIPE RDSRYSQPLH EEIALHKHLK HKNIVQYLGS FSENGFIKIF MEQVPGGSLS ALLRSKWGPL KDNEQTIGFY TKQILEGLKY LHDNQIVHRD IKGDNVLINT YSGVLKISDF GTSKRLAGIN PCTETFTGTL QYMAPEIIDK GPRGYGKAAD IWSLGCTIIE MATGKPPFYELGEPQAAMFK VGMFKVHPEI PESMSAEAKA FILKCFEPDP DKR
• Sequence Length: 1380

• Applicable Applications for ASK1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu693~Arg933 (Accession # O35099) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cytoplasm. Endoplasmic reticulum
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.6
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 171846249
• NCBI GeneID: 26408
• NCBI Accession #: NP_032606.4
• NCBI GenBank Nucleotide #: NM_008580.4
• UniProt Accession #: O35099; Q14AY5
• Molecular Weight: 30.8kDa
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 5
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 5
• NCBI Official Symbol: Map3k5
• NCBI Official Synonym Symbols: ASK; ASK1; Mekk5; MAPKKK5; 7420452D20Rik
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 5; ASK-1; MEKK 5; MEK kinase 5; MAPK/ERK kinase kinase 5; apoptosis signal-regulating kinase 1; mitogen activated protein kinase kinase kinase 5
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 5
• Protein Family: DASH complex
• UniProt Gene Name: Map3k5
• UniProt Synonym Gene Names: Ask1; Mekk5; ASK-1; MEK kinase 5; MEKK 5
• UniProt Entry Name: M3K5_MOUSE

Uniprot Description

ASK1: a MAP kinase kinase kinase of the STE11 family, apoptosis signal-regulating kinase 1 (ASK1) plays essential roles in stress-induced apoptosis. Activated by various stressors, including oxidative stress, endoplasmic reticulum stress, and calcium overload, as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) and lipopolysaccharide (LPS). In turn, ASK1 phosphorylates and activates two different subgroups of MAP kinase kinases, MKK4/SEK1 and MKK3/MKK6, which in turn activate stress-activated protein kinases (JNKs) and p38 subgroups of MAP kinases, respectively. Overexpression activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation as well as cell death. Oxidative stress induces the activation of ASK1, which correlate with ASK1 activity and ASK1-dependent apoptosis. Required for the innate immune response, which is essential for host defense against a wide range of pathogens.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; Protein kinase, STE; STE group; STE11 family

Cellular Component: endoplasmic reticulum; cytoplasm; intracellular

Molecular Function: protein homodimerization activity; metal ion binding; nucleotide binding; magnesium ion binding; caspase activator activity; protein kinase binding; protein phosphatase binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding

Biological Process: caspase activation; positive regulation of JNK activity; activation of MAPKK activity; activation of MAPK activity; apoptosis; immune system process; positive regulation of apoptosis; MAPKKK cascade; positive regulation of caspase activity; protein amino acid phosphorylation; JNK cascade; innate immune response; positive regulation of protein amino acid phosphorylation; induction of apoptosis by oxidative stress; phosphorylation